Aqueous phase separation in giant vesicles

被引：80

作者：

Helfrich, MR ^{[1
]}

Mangeney-Slavin, LK ^{[1
]}

Long, MS ^{[1
]}

Djoko, Y ^{[1
]}

Keating, CD ^{[1
]}

机构：

[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 45期

关键词：

D O I：

10.1021/ja028157+

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the synthesis and initial characterization of ∼10 μm diameter lipid vesicles that contain two distinct aqueous phases. The aqueous two-phase system is a dextran/poly(ethylene glycol) solution that exhibits temperature-dependent phase behavior. Vesicles were prepared above the phase transition temperature of the polymer solution. Upon cooling to room temperature, the polymer solution phase separated both within the vesicles and in the bulk solution. The location of poly(ethylene glycol)-rich and dextran-rich phases was determined by fluorescence microscopy. These structures are exciting in that they enable for the first time the interior volume of liposomes to be structured. Copyright © 2002 American Chemical Society.

引用

页码：13374 / 13375

页数：2

共 50 条

[21] Spontaneous Formation of Giant Unilamellar Vesicles from Microdroplets of a Polyion Complex by Thermally Induced Phase Separation
Oana, Hidehiro
Kishimura, Akihiro
Yonehara, Kei
Yamasaki, Yuichi
Washizu, Masao
Kataoka, Kazunori
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (25) : 4613 - 4616
[22] Modulation of phase separation at the micron scale and nanoscale in giant polymer/lipid hybrid unilamellar vesicles (GHUVs)
Thi Phuong Tuyen Dao
Fernandes, Fabio
Ibarboure, Emmanuel
Ferji, Khalid
Prieto, Manuel
Sandre, Olivier
Le Meins, Jean-Francois
SOFT MATTER, 2017, 13 (03) : 627 - 637
[23] Phase separation and curvature generation in biphasic giant lipid vesicles reconstituting plant plasma membrane lipids
Emami, Shiva
Ngassam, Viviane
Parikh, Atul
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2017, 253
[24] Phase behavior of phosphatidylethanolamines in giant unilamellar vesicles
Lakhani, Heena R.
Keller, Sarah L.
BIOPHYSICAL JOURNAL, 2007, : 234A - 234A
[25] Large-scale fluid/fluid phase separation of proteins and lipids in giant plasma membrane vesicles
Baumgart, Tobias
Hammond, Adam T.
Sengupta, Prabuddha
Hess, Samuel T.
Holowka, David A.
Baird, Barbara A.
Webb, Watt W.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (09) : 3165 - 3170
[26] Wrinkling and electroporation of giant vesicles in the gel phase
Knorr, Roland L.
Staykova, Margarita
Gracia, Ruben Serral
Dimova, Rumiana
SOFT MATTER, 2010, 6 (09) : 1990 - 1996
[27] Cholesterol-dependent phase separation in cell-derived giant plasma-membrane vesicles
Levental, Ilya
Byfield, Fitzroy J.
Chowdhury, Pramit
Gai, Feng
Baumgart, Tobias
Janmey, Paul A.
BIOCHEMICAL JOURNAL, 2009, 424 : 163 - 167
[28] MPP1 as a Factor Regulating Phase Separation in Giant Plasma Membrane-Derived Vesicles
Podkalicka, Joanna
Biernatowska, Agnieszka
Majkowski, Michal
Grzybek, Michal
Sikorski, Aleksander F.
BIOPHYSICAL JOURNAL, 2015, 108 (09) : 2201 - 2211
[29] A landscape of phase domains decorates giant vesicles
Brennan, M
CHEMICAL & ENGINEERING NEWS, 1999, 77 (32) : 39 - 39
[30] SEPARATION OF MEMBRANE-VESICLES BY AQUEOUS BIPHASIC PARTITIONING
ALBERTSSON, PA
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1994, 207 : 1 - IEC

← 1 2 3 4 5 →