Bioactive sesquiterpene derivatives from mangrove endophytic fungus Phomopsis sp. SYSU-QYP-23: Structures and nitric oxide inhibitory activities

被引：8

作者：

Chen, Yan ^{[1
,2
]}

Liu, Hongju ^{[3
]}

Zou, Ge ^{[1
]}

Yang, Wencong ^{[1
]}

Zhang, Lishan ^{[3
]}

Yan, Zhangyuan ^{[4
]}

Long, Yuhua ^{[4
]}

She, Zhigang ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Sch Chem, Guangzhou 510275, Peoples R China

[2] Henan Univ, Natl R&D Ctr Edible Fungus Proc Technol, Kaifeng 475004, Peoples R China

[3] Guangdong Med Univ, Sch Pharm, Dongguan 523808, Peoples R China

[4] South China Normal Univ, Sch Chem, Guangzhou 510006, Peoples R China

来源：

BIOORGANIC CHEMISTRY | 2021年 / 107卷

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

Phomopsis sp; Mangrove endophytic fungus; Sesquiterpene derivatives; Anti-inflammatory activity; Molecular docking;

D O I：

10.1016/j.bioorg.2020.104530

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eight new sesquiterpene derivatives (2, 4-6 and 10-13), along with five known analogues were isolated from the mangrove endophytic fungus Phomopsis sp. SYSU-QYP-23. Their structures of new compounds were established by spectroscopic methods, and the absolute configurations were confirmed by single-crystal X-ray diffraction analysis and comparison of the experimental ECD spectra. The absolute configuration of the side chain in 1 was first defined by modified Mosher's method. Compounds 1-7 showed potent inhibitory activities against nitric oxide (NO) production in lipopolysaccharides (LPS) induced RAW 264.7 cells with IC50 values ranging from 8.6 to 14.5 mu M. The molecular docking results implied that the bioactive sesquiterpenes may directly bind with targeting residues in the active cavity of iNOS protein.

引用

页数：9