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Structural studies of an immunoenhancing water-soluble glucan isolated from hot water extract of an edible mushroom, Pleurotus florida, cultivar Assam Florida
被引:54
|作者:
Roy, Sadhan K.
[1
]
Das, Debsankar
[1
]
Mondal, Subhas
[1
]
Maiti, Debabrata
[1
]
Bhunia, Bibhas
[2
]
Maiti, Tapas K.
[2
]
Islam, Syed S.
[1
]
机构:
[1] Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India
[2] Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India
关键词:
Pleurotus florida;
cv Assam Florida;
Mushroom polysaccharide;
Structure;
NMR spectroscopy;
Immunoactivation;
SAJOR-CAJU;
AQUEOUS EXTRACT;
ANTITUMOR POLYSACCHARIDES;
CHINESE MUSHROOM;
(1->3)-BETA-D-GLUCAN;
ELUCIDATION;
BODIES;
D O I:
10.1016/j.carres.2009.09.010
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
An immunoenhancing polysaccharide isolated from the hot water extract of the fruiting bodies of an edible mushroom Pleurotus florida, cultivar Assam Florida, was found to consist of only D-glucose as a monosaccharide constituent. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, Smith degradation, and NMR experiments (H-1, C-13, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of the polysaccharide was established as [GRAPHICS] This glucan stimulates macrophages, splenocytes, and thymocytes. (C) 2009 Elsevier Ltd. All rights reserved.
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页码:2596 / 2601
页数:6
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