Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells

被引：25

作者：

Wei, Yu-Dong ^{[1
]}

Du, Xiao-Min ^{[1
]}

Yang, Dong-Hui ^{[1
]}

Ma, Fang-Lin ^{[1
]}

Yu, Xiu-Wei ^{[1
]}

Zhang, Meng-Fei ^{[1
]}

Li, Na ^{[1
]}

Peng, Sha ^{[1
]}

Liao, Ming-Zhi ^{[2
]}

Li, Guang-Peng ^{[3
]}

Bai, Chun-Ling ^{[3
]}

Liu, Wei-Shuai ^{[4
]}

Hua, Jin-Lian ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China

[2] Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China

[3] Inner Mongolia Univ, Sch Life Sci, State Key Lab Reprod Regulat & Breeding Grassland, Hohhot 010021, Inner Mongolia, Peoples R China

[4] Yangling Demonstrat Zone Hosp, Dept Pathol, Yangling 712100, Shaanxi, Peoples R China

来源：

ZOOLOGICAL RESEARCH | 2021年 / 42卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Male germline stem cells (mGSCs); Goat; Dmrt1; Plzf; Immune response; TOLL-LIKE RECEPTORS; MOUSE SERTOLI; SPERMATOGENIC CELLS; MALE-INFERTILITY; SEX REVERSAL; PLZF; EXPRESSION; PROLIFERATION; TRANSCRIPTION; ACTIVATION;

D O I：

10.24272/j.issn.2095-8137.2020.186

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Double sex and mab-3-related transcription factor 1 (Dmrt1), which is expressed in goat male germline stem cells (mGSCs) and Sertoli cells, is one of the most conserved transcription factors involved in sex determination. In this study, we highlighted the role of Dmrt1 in balancing the innate immune response in goat mGSCs. Dmrt1 recruited promyelocytic leukemia zinc finger (Plzf), also known as zinc finger and BTB domain-containing protein 16 (Zbtb16), to repress the Toll-like receptor 4 (TLR4)-dependent inflammatory signaling pathway and nuclear factor (NF)-kappa B. Knockdown of Dmrt1 in seminiferous tubules resulted in widespread degeneration of germ and somatic cells, while the expression of proinflammatory factors were significantly enhanced. We also demonstrated that Dmrt1 stimulated proliferation of mGSCs, but repressed apoptosis caused by the immune response. Thus, Dmrt1 is sufficient to reduce inflammation in the testes, thereby establishing the stability of spermatogenesis and the testicular microenvironment.

引用

页码：14 / 27

页数：14

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