Ribosomal DNA contributes to global chromatin regulation

被引:113
作者
Paredes, Silvana [1 ]
Maggert, Keith A. [1 ]
机构
[1] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA
基金
美国国家卫生研究院;
关键词
Drosophila; epigenetics; heterochromatin; nucleolus; rDNA; POSITION-EFFECT VARIEGATION; EXTRACHROMOSOMAL CIRCULAR DNA; REPEATED GENOMIC SEQUENCES; GENE-EXPRESSION; BOBBED MUTANTS; X-CHROMOSOME; Y-CHROMOSOME; DROSOPHILA; RDNA; NUCLEOLUS;
D O I
10.1073/pnas.0906811106
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The 35S ribosomal RNA genes (rDNA) are organized as repeated arrays in many organisms. Epigenetic regulation of transcription of the rRNA results in only a subset of copies being transcribed, making rDNA an important model for understanding epigenetic chromatin modification. We have created an allelic series of deletions within the rDNA array of the Drosophila Y chromosome that affect nucleolus size and morphology, but do not limit steady-state rRNA concentrations. These rDNA deletions result in reduced heterochromatin-induced gene silencing elsewhere in the genome, and the extent of the rDNA deletion correlates with the loss of silencing. Consistent with this, chromosomes isolated from strains mutated in genes required for proper heterochromatin formation have very small rDNA arrays, reinforcing the connection between heterochromatin and the rDNA. In wild-type cells, which undergo spontaneous natural rDNA loss, we observed the same correlation between loss of rDNA and loss of heterochromatin-induced silencing, showing that the volatility of rDNA arrays may epigenetically influence gene expression through normal development and differentiation. We propose that the rDNA contributes to a balance between heterochromatin and euchromatin in the nucleus, and alterations in rDNA-induced or natural-affect this balance.
引用
收藏
页码:17829 / 17834
页数:6
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