Evaluation of RE and B1 Genes as Targets for Detection of Toxoplasma gondii by Nested PCR in Blood Samples of Patients with Ocular Toxoplasmosis

被引：1

作者：

Esboei, Bahman Rahimi ^{[1
]}

Kazemi, Bahram ^{[2
]}

Zarei, Mohammad ^{[3
]}

Mohebali, Mehdi ^{[1
]}

Valian, Hossein Keshavarz ^{[1
]}

Shojaee, Saeedeh ^{[1
]}

Zahedipour, Fatemeh ^{[2
]}

Fallahi, Shirzad ^{[4
]}

Mousavi, Parisa ^{[1
]}

Mahmoudzadeh, Raziyeh ^{[3
]}

Salabati, Mirataollah ^{[3
]}

机构：

[1] Univ Tehran Med Sci, Sch Publ Hlth, Dept Med Parasitol & Mycol, Tehran, Iran

[2] Shahid Beheshti Univ Med Sci, Cellular & Mol Biol Res Ctr, Tehran, Iran

[3] Univ Tehran Med Sci, Farabi Eye Hosp, Eye Res Ctr, Tehran, Iran

[4] Lorestan Univ Med Sci, Dept Med Parasitol & Mycol, Khorramabad, Iran

来源：

ACTA PARASITOLOGICA | 2019年 / 64卷 / 02期

关键词：

Toxoplasma gondii; Ocular toxoplasmosis; Nested PCR; RE gene; B1; gene; EPIDEMIOLOGY; INFECTIONS; DIAGNOSIS;

D O I：

10.2478/s11686-019-00056-6

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

IntroductionTo evaluate B1 and RE genes as targets to detect Toxoplasma gondii, nested PCR is used in blood samples of patients with ocular toxoplasmosis.Materials and MethodsFollowing the measurement of IgG and IgM antibodies using indirect ELISA, IgG avidity and assessment of blood samples by nested PCR, the agreement between various test results was studied.ResultsFrom 117 patients, 77 (65.81%) were found to be positive for IgG anti-Toxoplasma antibody, 12 cases were positive for both IgG and IgM, and 1 patient was positive for IgM only. The detection limit for the RE-nested PCR assay was one T. gondii tachyzoite, whereas the limit for B1-nested PCR was five tachyzoites. Nested PCR results showed higher agreement with IgM test results than IgG test results.ConclusionThe results of this study showed that nested PCR of peripheral blood is a useful and non-invasive method for detection of T. gondii in patients with OT, especially in case of recently acquired infections, and RE targeted assay is more sensitive than B1 targeted assay for this purpose.

引用

页码：384 / 389

页数：6

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