Interleukin-17 regulates chemokine and gelatinase B expression in fibroblasts to recruit both neutrophils and monocytes

被引：36
作者：
Qiu, Zheng
Dillen, Chris
Hu, Jialiang
Verbeke, Hannelien
Struyf, Sofie
Van Damme, Jo
Opdenakker, Ghislain [1 ]
机构：
[1] Univ Louvain, Rega Inst Med Res, Immunobiol Lab, B-3000 Louvain, Belgium
来源：
IMMUNOBIOLOGY | 2009年 / 214卷 / 9-10期
关键词：
Chemokine; Fibroblast; Monocyte chemotactic protein-1; Interleukin-17; Matrix metalloproteinase; Tissue inhibitor of metalloprotease; IFN-GAMMA; RHEUMATOID-ARTHRITIS; MICROARRAY ANALYSIS; T-CELLS; INFLAMMATION; IL-17; MATRIX-METALLOPROTEINASE-9; STIMULATION; DESTRUCTION; RECEPTORS;
D O I：
10.1016/j.imbio.2009.06.007
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Interleukin 17 (IL-17) is a proinflammatory cytokine, produced only by activated lymphocytes, but with a broad cellular host range. The effects of IL-17 on fibroblasts were investigated by analysis of the induction of chemokine and matrix metalloprotease (MMP) mRNA levels by RT-PCR. IL-17 stimulated CC chemokine (monocyte chemotactic protein-1; MCP-1/JE) and CXC (KC, MIP-2) chemokine and TIMP-1 mRNA expression in fibroblastoid L929 cells. In normal mouse embryonic fibroblasts (MEF) this induction profile by IL-17 was extended with the mRNAs encoding the chemokine granulocyte chemotactic protein-2 (GCP-2) and the proteases MMP-3, MMP-9 and MMP-13. The MMP-9 and GCP-2 induction by IL-17 in MEF, and the absence of induction in L929 cells, were corroborated by gelatin zymography and ELISA, respectively. The induction of MCP-1/CCL2 by IL-17 was confirmed in human diploid fibroblasts. We conclude that IL-17 regulates differentially chemokine and MMP expression by normal and transformed fibroblasts and is indirectly capable of attracting both monocytes and neutrophils to the inflammatory focus. (C) 2009 Elsevier GmbH. All rights reserved.
引用
收藏
页码：835 / 842
页数：8
相关论文
共 29 条
[1]   Interleukin-17 in sputum correlates with airway hyperresponsiveness to methacholine [J].
Barczyk, A ;
Pierzchala, W ;
Sozañska, E .
RESPIRATORY MEDICINE, 2003, 97 (06) :726-733
[2]   Contribution of interleukin 17 to synovium matrix destruction in rheumatoid arthritis [J].
Chabaud, M ;
Garnero, P ;
Dayer, JM ;
Guerne, PA ;
Fossiez, F ;
Miossec, P .
CYTOKINE, 2000, 12 (07) :1092-1099
[3]   Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain [J].
Cua, DJ ;
Sherlock, J ;
Chen, Y ;
Murphy, CA ;
Joyce, B ;
Seymour, B ;
Lucian, L ;
To, W ;
Kwan, S ;
Churakova, T ;
Zurawski, S ;
Wiekowski, M ;
Lira, SA ;
Gorman, D ;
Kastelein, RA ;
Sedgwick, JD .
NATURE, 2003, 421 (6924) :744-748
[4]   Analysis of gelatinases in complex biological fluids and tissue extracts [J].
Descamps, FJ ;
Martens, E ;
Opdenakker, G .
LABORATORY INVESTIGATION, 2002, 82 (11) :1607-1608
[5]   Increased expression of interleukin 17 in inflammatory bowel disease [J].
Fujino, S ;
Andoh, A ;
Bamba, S ;
Ogawa, A ;
Hata, K ;
Araki, Y ;
Bamba, T ;
Fujiyama, Y .
GUT, 2003, 52 (01) :65-70
[6]   Functional relevance of the IL-23-IL-17 axis in lungs in vivo [J].
Ivanov, Stefan ;
Bozinovski, Steven ;
Bossios, Apostolos ;
Valadi, Hadi ;
Vlahos, Ross ;
Malmhall, Carina ;
Sjostrand, Margareta ;
Kolls, Jay K. ;
Anderson, Gary P. ;
Linden, Anders .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2007, 36 (04) :442-451
[7]  
Jovanovic DV, 2000, ARTHRITIS RHEUM-US, V43, P1134, DOI 10.1002/1529-0131(200005)43:5<1134::AID-ANR24>3.0.CO
[8]  
2-#
[9]   Protective role of IFN-γ in collagen-induced arthritis conferred by inhibition of mycobacteria-induced granulocyte chemotactic protein-2 production [J].
Kelchtermans, Hilde ;
Struyf, Sofie ;
De Klerck, Bert ;
Mitera, Tania ;
Alen, Marijke ;
Geboes, Lies ;
Van Balen, Maarten ;
Dillen, Chris ;
Put, Willy ;
Gysemans, Conny ;
Billiau, Alfons ;
Van Damme, Jo ;
Matthys, Patrick .
JOURNAL OF LEUKOCYTE BIOLOGY, 2007, 81 (04) :1044-1053
[10]   Interleukin-17 receptor deficiency results in impaired synovial expression of interleukin-1 and matrix metalloproteinases 3, 9, and 13 and prevents cartilage destruction during chronic reactivated streptococcal cell wall-induced arthritis [J].
Koenders, MI ;
Kolls, JK ;
Oppers-Walgreen, B ;
van den Bersselaar, L ;
Joosten, LAB ;
Schurr, JR ;
Schwarzenberger, P ;
van den Berg, WB ;
Lubberts, E .
ARTHRITIS AND RHEUMATISM, 2005, 52 (10) :3239-3247
123