Gene Expression Profile of Human Skeletal Muscle and Adipose Tissue of Chinese Han Patients with Type 2 Diabetes Mellitus

被引：11
作者：
Yang, Yan-Li [3 ,4 ]
Xiang, Ruo-Lan [5 ]
Yang, Chang [1 ,2 ]
Liu, Xiao-Jun [1 ,2 ]
Shen, Wen-Jun [6 ,7 ]
Zuo, Jin [1 ,2 ]
Chang, Yong-Sheng [1 ,2 ]
Fang, Fu-De [1 ,2 ]
机构：
[1] Peking Union Med Coll, Inst Basic Med Sci, Natl Lab Med Mol Biol, Beijing 100005, Peoples R China
[2] Chinese Acad Med Sci, Beijing 100005, Peoples R China
[3] Beijing Union Med Coll Hosp, Peking Union Med Coll, Dept Internal Med, Beijing 100730, Peoples R China
[4] Chinese Acad Med Sci, Beijing 100730, Peoples R China
[5] Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
[6] Stanford Univ, Div Endocrinol, Palo Alto, CA 94304 USA
[7] VA Palo Alto Hlth Care Syst, Palo Alto, CA 94304 USA
来源：
BIOMEDICAL AND ENVIRONMENTAL SCIENCES | 2009年 / 22卷 / 05期
基金：
国家科技攻关计划; 国家高技术研究发展计划(863计划);
关键词：
Type 2 diabetes mellitus (T2DM); Microarray technology; Skeletal muscle; Adipose tissue; TYROSINE PHOSPHATASE-1B GENE; M2-TYPE PYRUVATE-KINASE; INSULIN SENSITIVITY; GLUCOSE; ASSOCIATION; MICROARRAY; RESISTANCE; PATTERNS; OBESITY; PTPN1;
D O I：
10.1016/S0895-3988(10)60012-8
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Objective To study the differential patterns of gene expression in skeletal muscle and adipose tissue between type 2 diabetes mellitus (T2DM) patients and healthy subjects using DNA microarray analysis. Methods T2DM patiens were divided into female group, young male group and old male group. DNA microarray analysis and quantitative real-time PCR were carried out to analyze the relation between gene expressions and T2DM. Results The mRNA expression of 298, 578, and 350 genes was changed in the skeletal muscle of diabetes mellitus patients compared with control subjects. The 1320, 1143, and 2847 genes were modified in adipose tissue of the three groups. Among the genes surveyed, the change of 25 and 39 gene transcripts in skeletal muscle and adipose tissue was >= 2 folds. These differentially expressed genes were classified into 15 categories according to their functions. Conclusion New genes are found and T2DM can be prevented or cured.
引用
收藏
页码：359 / 368
页数：10
相关论文
共 24 条
[1]  
[Anonymous], TECHN REP SER WHO
[2]  
ASHIZAWA K, 1991, J BIOL CHEM, V266, P16842
[3]  
Bennett PH, 1999, NUTR REV, V57, pS51, DOI 10.1111/j.1753-4887.1999.tb01788.x
[4]   Making and reading microarrays [J].
Cheung, VG ;
Morley, M ;
Aguilar, F ;
Massimi, A ;
Kucherlapati, R ;
Childs, G .
NATURE GENETICS, 1999, 21 (Suppl 1) :15-19
[5]   Cluster analysis and display of genome-wide expression patterns [J].
Eisen, MB ;
Spellman, PT ;
Brown, PO ;
Botstein, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) :14863-14868
[6]   Increased insulin sensitivity and obesity resistance in mice lacking the protein tyrosine phosphatase-1B gene [J].
Elchebly, M ;
Payette, P ;
Michaliszyn, E ;
Cromlish, W ;
Collins, S ;
Loy, AL ;
Normandin, D ;
Cheng, A ;
Himms-Hagen, J ;
Chan, CC ;
Ramachandran, C ;
Gresser, MJ ;
Tremblay, ML ;
Kennedy, BP .
SCIENCE, 1999, 283 (5407) :1544-1548
[7]   Association testing of the protein tyrosine phosphatase 1B gene (PTPN1) with type 2 diabetes in 7,883 people [J].
Florez, JC ;
Agapakis, CM ;
Burtt, NP ;
Sun, M ;
Almgren, P ;
Råstam, L ;
Tuomi, T ;
Gaudet, D ;
Hudson, TJ ;
Daly, MJ ;
Ardlie, KG ;
Hirschhorn, JN ;
Groop, L ;
Altshuler, B .
DIABETES, 2005, 54 (06) :1884-1891
[8]   Interaction between HERC1 and M2-type pyruvate kinase [J].
Garcia-Gonzalo, FR ;
Cruz, C ;
Muñoz, P ;
Mazurek, S ;
Eigenbrodt, E ;
Ventura, F ;
Bartrons, R ;
Rosa, JL .
FEBS LETTERS, 2003, 539 (1-3) :78-84
[9]   IGF alters jejunal glucose transporter expression and serum glucose levels in immature rats [J].
Lane, RH ;
Dvorak, B ;
MacLennan, NK ;
Dvorakova, K ;
Halpern, MD ;
Pham, TD ;
Philipps, AF .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2002, 283 (06) :R1450-R1460
[10]   High glucose alters connexin 43 expression and gap junction intercellular communication activity in retinal pericytes [J].
Li, AF ;
Sato, T ;
Haimovici, R ;
Okamoto, T ;
Roy, S .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2003, 44 (12) :5376-5382
123