Prevalence of microsporidia, Cryptosporidium spp., and Giardia spp. in beavers (Castor canadensis) in Massachusetts

被引：41

作者：

Fayer, Ronald ^{[1
]}

Santin, Monica

Trout, James M.

DeStefano, Stephen

Koenen, Kiana

Kaur, Taranjit

机构：

[1] USDA ARS, Environm Microbial Safety Lab, Anim & Nat Resources Inst, Beltsville, MD 20705 USA

[2] Univ Massachusetts, US Geol Survey, Massachusetts Cooperat Fish & Wildlife Res Unit, Amherst, MA 01355 USA

[3] Virginia Polytech Inst & State Univ, Virginia Maryland Reg Coll Vet Med, Blacksburg, VA 24061 USA

来源：

JOURNAL OF ZOO AND WILDLIFE MEDICINE | 2006年 / 37卷 / 04期

关键词：

beaver; genotype; molecular; survey; waterborne; zoonotic;

D O I：

10.1638/06-013.1

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Feces from 62 beavers (Castor canadensis) in Massachusetts were examined by fluorescence microscopy (IFA) and polymerase chain reaction (PCR) for Microsporidia species, Cryptosporidium spp., and Giardia spp. between January 2002 and December 2004. PCR-positive specimens were further examined by gene sequencing. Protist parasites were detected in 6.4% of the beavers. All were subadults and kits. Microsporidia species were not detected. Giardia spp. was detected by IFA from four beavers; Cryptosporidium spp. was also detected by IFA from two of these beavers. However, gene sequence data for the ssrRNA gene from these two Cryptosporidium spp.-positive beavers were inconclusive in identifying the species. Nucleotide sequences of the TPI, ssrRNA, and P-giardin genes for Giardia spp. (deposited in GenBank) indicated that the four beavers were excreting Giardia duodenalis Assemblage B, the zoonotic genotype representing a potential source of waterborne Giardia spp. cysts.

引用

页码：492 / 497

页数：6

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