Is raw better? A multiple DNA barcoding approach (full and mini) based on mitochondrial and nuclear markers reveals low rates of misdescription in sushi products sold on the Italian market

被引:37
作者
Armani, A. [1 ]
Tinacci, L. [1 ]
Lorenzetti, R. [2 ]
Benvenuti, A. [1 ]
Susini, F. [3 ]
Gasperetti, L. [3 ]
Ricci, E. [3 ]
Guarducci, M. [3 ]
Guidi, A. [1 ]
机构
[1] Univ Pisa, Dept Vet Sci, FishLab, Viale Piagge 2, I-56124 Pisa, Italy
[2] Expt Zooprophylact Inst Lazio & Tuscany, Via Appia Nuova, Rome, Italy
[3] Expt Zooprophylact Inst Lazio & Tuscany, SS Abetone & Brennero 4, I-56123 Pisa, Italy
关键词
Sushi products; DNA barcoding; COI gene; 16S rRNA gene; PEPCK gene; Misdescription; PROTEIN-CODING GENES; 16S RIBOSOMAL-RNA; SPECIES IDENTIFICATION; SEAFOOD; FISH; FOOD; PHYLOGENY; AUTHENTICATION; EXTRACTION; BIVALVIA;
D O I
10.1016/j.foodcont.2017.03.030
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
New dietary habits have favored an ever growing popularity of Eastern country cooking style and in particular of sushi. Even though the Reg. (EU) 1379/2013 does not apply to restaurants and caterers, the Reg. (EU) 1169/2011 establishes that all the information they provided to the final consumer have to meet the transparency requirements as regards the description of the ingredients used for the preparation of food. The present study aimed at performing a molecular based survey to identify the seafood species used in the sushi preparations at the retail level. A total of 185 samples were collected from sushi venues and supermarkets and DNA barcoding, followed by a pairwise divergence and Neighbor Joining clustering analysis, was applied in order to verify the information declared at purchase. Rather than to a proper training of Food Business Operators working at the catering level, the low mislabeling rate found in this study (3.4%) could be ascribed to the standardization of the products sold in ethnic restaurants. In fact, the common practice of proposing standardized menus always relying on the same species of fish could limit the risk of mislabeling occurrence. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码:126 / 133
页数:8
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