Spliceosomal snRNA Epitranscriptomics

被引：69
作者：
Morais, Pedro [1 ]
Adachi, Hironori [2 ]
Yu, Yi-Tao [2 ]
机构：
[1] ProQR Therapeut, Leiden, Netherlands
[2] Univ Rochester, Med Ctr, Dept Biochem & Biophys, Ctr RNA Biol, Rochester, NY 14642 USA
来源：
FRONTIERS IN GENETICS | 2021年 / 12卷
基金：
美国国家卫生研究院;
关键词：
pre-mRNA splicing; small nuclear RNA; RNA modifications; epitranscriptomics; pseudouridine; 2' -O-methylation; N6-methyladenosine; N2-methylation; SMALL NUCLEAR RNAS; PSEUDOURIDYLATION GUIDE RNA; PRIMARY NUCLEOTIDE-SEQUENCE; PRECURSOR MESSENGER-RNA; U4/U6.U5; TRI-SNRNP; YEAST U2 SNRNA; BRANCH-SITE; CROSS-LINKING; U6; SNRNA; POSTTRANSCRIPTIONAL MODIFICATIONS;
D O I：
10.3389/fgene.2021.652129
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Small nuclear RNAs (snRNAs) are critical components of the spliceosome that catalyze the splicing of pre-mRNA. snRNAs are each complexed with many proteins to form RNA-protein complexes, termed as small nuclear ribonucleoproteins (snRNPs), in the cell nucleus. snRNPs participate in pre-mRNA splicing by recognizing the critical sequence elements present in the introns, thereby forming active spliceosomes. The recognition is achieved primarily by base-pairing interactions (or nucleotide-nucleotide contact) between snRNAs and pre-mRNA. Notably, snRNAs are extensively modified with different RNA modifications, which confer unique properties to the RNAs. Here, we review the current knowledge of the mechanisms and functions of snRNA modifications and their biological relevance in the splicing process.
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页数：16
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