The rectal mucosal but not fecal microbiota detects subclinical ulcerative colitis

被引:7
作者
Lin, Yu-Fei [1 ]
Sung, Chang Mu [2 ,3 ]
Ke, Huei-Mien [1 ]
Kuo, Chia-Jung [2 ]
Liu, Wei-an [1 ]
Tsai, Wen-Sy [4 ]
Lin, Cheng-Yu [2 ]
Cheng, Hao-Tsai [2 ]
Lu, Meiyeh J. [1 ]
Tsai, Isheng J. [1 ]
Hsieh, Sen-Yung [2 ,5 ]
机构
[1] Acad Sinica, Biodivers Res Ctr, Taipei, Taiwan
[2] Chang Gung Mem Hosp, Dept Gastroenterol & Hepatol, Taoyuan, Taiwan
[3] Chang Gung Univ, Grad Inst Clin Med Sci, Taoyuan, Taiwan
[4] Chang Gung Univ, Chang Gung Mem Hosp, Div Colorectal Surg, Taoyuan, Taiwan
[5] Chang Gung Univ, Coll Med, Grad Inst Biomed Sci, Taoyuan, Taiwan
关键词
Gut microbiota; inflammatory bowel disease; rectal biopsy microbiome; ulcerative colitis; subclinical UC detection; INFLAMMATORY-BOWEL-DISEASE; GUT MICROBIOME; DYSBIOSIS; PATHOGENESIS; PREVALENCE; DIVERSITY; INDEX; ACIDS;
D O I
10.1080/19490976.2020.1832856
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Ulcerative colitis (UC), a subtype of inflammatory bowel disease, is characterized by repetitive remission and relapse. Gut microbiome is critically involved in pathogenesis of UC. The shifts in microbiome profile during disease remission remain under-investigated. Recent studies revealed that UC pathogenesis is likely to originate in the mucosal barrier. Therefore, we investigated the effectiveness of mucosal tissue microbiomes to differentiate patients with subclinical UC from healthy individuals. The microbiomes of cecal and rectal biopsies and feces were characterized from 13 healthy individuals and 45 patients with subclinical UC. Total genomic DNA was extracted from the samples, and their microbial communities determined using next-generation sequencing. We found that changes in relative abundance of subclinical UC were marked by a decrease in Proteobacteria and an increase in Bacteroidetes phyla in microbiome derived from rectal tissues but not cecal tissue nor feces. Only in the microbiome of rectal tissue had significantly higher community richness and evenness in subclinical UC patients than controls. Twenty-seven operational taxonomic units were enriched in subclinical UC cohort with majority of the taxa from the Firmicutes phylum. Inference of putative microbial functional pathways from rectal biopsy microbiome suggested a differential increase in interleukin-17 signaling and T-helper cell differentiation pathways. Rectal biopsy tissue was suggested to be more suitable than fecal samples for microbiome assays to distinguish patients with subclinical UC from healthy adults. Assessment of the rectal biopsy microbiome may offer clinical insight into UC disease progression and predict relapse of the diseases.
引用
收藏
页码:1 / 10
页数:10
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