Green synthesis of silver nanoparticles via Cynara scolymus leaf extracts: The characterization, anticancer potential with photodynamic therapy in MCF7 cells

被引:222
作者
Erdogan, Omer [1 ]
Abbak, Muruvvet [2 ]
Demirbolat, Gulen Melike [3 ]
Birtekocak, Fatih [1 ]
Aksel, Mehran [4 ]
Pasa, Salih [5 ]
Cevik, Ozge [1 ,2 ]
机构
[1] Adnan Menderes Univ, Sch Med, Dept Biochem, Aydin, Turkey
[2] Adnan Menderes Univ, Sci Technol Res & Applicat Ctr, Aydin, Turkey
[3] Sivas Cumhuriyet Univ, Dept Pharmaceut Technol, Fac Pharm, Sivas, Turkey
[4] Aydin Adnan Univ, Sch Med, Dept Biophys, Aydin, Turkey
[5] Afyon Kocatepe Univ, Dept Sci, Fac Educ, Afyon, Turkey
关键词
SKIN-CANCER; ARTICHOKE; ANTIOXIDANT; METAL;
D O I
10.1371/journal.pone.0216496
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In this study, we report on the synthesis of silver nanoparticles (AgNPs) from the leaf extracts of Cynara scolymus (Artichoke) using microwave irradiation and the evaluation of its anti-cancer potential with photodynamic therapy (PDT). Silver nanoparticles formation was characterized by scanning electron microscopy with energy dispersive x-ray spectroscopy and Fourier transform infrared (FTIR) spectroscopy. Silver nanoparticles formation was also investigated the surface charge, particle size and distribution using zetasizer analysis. The cytotoxic effect of AgNPs and/or PDT was studied by MTT assay and migration by the scratch assay. The apoptotic inducing ability of the AgNPs and/or PDT was investigated by intracellular ROS analysis, antioxidant enzyme levels (SOD, CAT, GPx and GSH), Hoechst staining and Bax/Bcl-2 analysis using western blotting. The mean particle size of produced AgNPs was found 98.47 +/- 2.04 nm with low polydispersity (0.301 +/- 0.033). Zeta potential values of AgNPs show -32.3 +/- 0.8 mV. These results clearly indicate the successful formation of AgNPs for cellular uptake. Mitochondrial damage and intracellular ROS production were observed upon treatment with AgNPs (10 mu g/mL) and PDT (0.5 mJ/cm(2)) showed significant reducing cell migration, expression of Bax and suppression of Bcl-2. Significantly, biosynthesized AgNPs showed a broad-spectrum anti-cancer activity with PDT therapy and therefore represent promoting ROS generation by modulating mitochondrial apoptosis induction in MCF7 breast cancer cells.
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页数:15
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