A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging

被引：323
作者：
Specht, Elizabeth A. [1 ,2 ]
Braselmann, Esther [1 ,2 ]
Palmer, Amy E. [1 ,2 ]
机构：
[1] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80303 USA
[2] Univ Colorado, BioFrontiers Inst, Boulder, CO 80303 USA
来源：
ANNUAL REVIEW OF PHYSIOLOGY, VOL 79 | 2017年 / 79卷
关键词：
fluorescent proteins; fluorescent dyes; probes; live-cell imaging; cellular dynamics; FLUOROGENIC CYANINE DYES; HIGH-CONTENT SCREENS; LIVING HUMAN-CELLS; IN-VIVO; SINGLE-CELL; PROTEIN LOCALIZATION; SACCHAROMYCES-CEREVISIAE; AMINO-ACID; DNA-DAMAGE; SUPERRESOLUTION MICROSCOPY;
D O I：
10.1146/annurev-physiol-022516-034055
中图分类号：
Q4 [生理学];
学科分类号：
071003 ;
摘要：
Fluorescent tools have revolutionized our ability to probe biological dynamics, particularly at the cellular level. Fluorescent sensors have been developed on several platforms, utilizing either small-molecule dyes or fluorescent proteins, to monitor proteins, RNA, DNA, small molecules, and even cellular properties, such as pH and membrane potential. We briefly summarize the impressive history of tool development for these various applications and then discuss the most recent noteworthy developments in more detail. Particular emphasis is placed on tools suitable for single-cell analysis and especially live-cell imaging applications. Finally, we discuss prominent areas of need in future fluorescent tool development-specifically, advancing our capability to analyze and integrate the plethora of high-content data generated by fluorescence imaging.
引用
收藏
页码：93 / 117
页数：25
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