Fish muscle parvalbumins as marker proteins for native and urea isoelectric focusing

被引：2
作者：
Rehbein, H
Kündiger, R
Pineiro, C
Perez-Martin, RI
机构：
[1] Fed Res Ctr Fisheries, Inst Biochem & Technol, D-22767 Hamburg, Germany
[2] CSIC, Inst Invest Marinas, Vigo, Spain
来源：
ELECTROPHORESIS | 2000年 / 21卷 / 08期
关键词：
parvalbumins; isoelectric focusing; marker proteins;
D O I：
10.1002/(SICI)1522-2683(20000501)21:8<1458::AID-ELPS1458>3.3.CO;2-K
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An isoelectric point (pl) calibration kit containing fish muscle parvalbumins was prepared and tested for its suitability for isoelectric focusing (IEF) in the presence of 8 M urea. The pattern obtained by urea CleanGel IEF consisted of nine bands covering the pi range 4.96-5.64. This range is relevant for species identification of heated fish by urea IEF. The kit may also be used for native IEF in the low pH range, as demonstrated by running an extract made from the kit together with water-soluble fish muscle proteins on Servalyt Precotes 3-6.
引用
收藏
页码：1458 / 1463
页数：6
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