Fish muscle parvalbumins as marker proteins for native and urea isoelectric focusing

被引：2

作者：

Rehbein, H

Kündiger, R

Pineiro, C

Perez-Martin, RI

机构：

[1] Fed Res Ctr Fisheries, Inst Biochem & Technol, D-22767 Hamburg, Germany

[2] CSIC, Inst Invest Marinas, Vigo, Spain

来源：

ELECTROPHORESIS | 2000年 / 21卷 / 08期

关键词：

parvalbumins; isoelectric focusing; marker proteins;

D O I：

10.1002/(SICI)1522-2683(20000501)21:8<1458::AID-ELPS1458>3.3.CO;2-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An isoelectric point (pl) calibration kit containing fish muscle parvalbumins was prepared and tested for its suitability for isoelectric focusing (IEF) in the presence of 8 M urea. The pattern obtained by urea CleanGel IEF consisted of nine bands covering the pi range 4.96-5.64. This range is relevant for species identification of heated fish by urea IEF. The kit may also be used for native IEF in the low pH range, as demonstrated by running an extract made from the kit together with water-soluble fish muscle proteins on Servalyt Precotes 3-6.

引用

页码：1458 / 1463

页数：6

共 19 条

[1] SPECIES IDENTIFICATION OF RAW AND BOILED SHRIMP BY A UREA GEL ISOELECTRIC-FOCUSING TECHNIQUE [J].

AN, H ;

MARSHALL, MR ;

OTWELL, WS ;

WEI, CI .

JOURNAL OF FOOD SCIENCE, 1989, 54 (02) :233-+

[2] ELECTROPHORETIC IDENTIFICATION OF FISH SPECIES USED IN SURIMI PRODUCTS [J].

AN, H ;

WEI, CI ;

ZHAO, J ;

MARSHALL, MR ;

LEE, CM .

JOURNAL OF FOOD SCIENCE, 1989, 54 (02) :253-257

[3] TWO-DIMENSIONAL GEL-ELECTROPHORESIS OF PROTEINS [J].

DUNN, MJ .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 418 :145-185

[4]

Etienne M, 1999, ELECTROPHORESIS, V20, P1923, DOI 10.1002/(SICI)1522-2683(19990701)20:10<1923::AID-ELPS1923>3.0.CO

[5]

2-J

[6] ISOELECTRIC POINTS OF PROTEINS, DETERMINED BY ISOELECTRIC-FOCUSING IN THE PRESENCE OF UREA AND ETHANOL [J].

GELSEMA, WJ ;

DELIGNY, CL ;

VANDERVEEN, NG .

JOURNAL OF CHROMATOGRAPHY, 1979, 171 (APR) :171-181

[7]

GERDAY C, 1982, MOL PHYSIOL, V2, P63

[8] COMPARISON OF PARVALBUMIN PATTERNS FROM DIFFERENT FISH SPECIES BY ISOELECTRIC-FOCUSING OF MUSCLE EXTRACTS [J].

GIRIJA, N ;

REHBEIN, H .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1988, 91 (04) :723-728

[9] TWO-DIMENSIONAL GEL-ELECTROPHORESIS OF CHICKEN SKELETAL-MUSCLE PROTEINS WITH AGAROSE GELS IN THE 1ST DIMENSION [J].

HIRABAYASHI, T .

ANALYTICAL BIOCHEMISTRY, 1981, 117 (02) :443-451

[10] DETECTION OF COWS MILK IN SHEEPS AND GOATS MILK AND CHEESE BY ISOELECTRIC-FOCUSING ON THIN-LAYERS OF POLYACRYLAMIDE GELS CONTAINING UREA [J].

KRAUSE, I ;

BELITZ, HD ;

KAISER, KP .

ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG, 1982, 174 (03) :195-199

← 1 2 →