Comparative transcriptomic analysis of Bombyx mori fat body tissue following dietary restriction

被引:7
作者
Pan, Ye [1 ,2 ,3 ]
Lu, Peng [2 ]
Wang, Qinyun [1 ]
Zhu, Feifei [2 ]
Li, Chengjun [2 ]
He, Yuanqing [1 ,3 ]
Chen, Keping [2 ]
机构
[1] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang, Peoples R China
[2] Jiangsu Univ, Inst Life Sci, Zhenjiang, Peoples R China
[3] Jiangsu Univ, Lab Anim Res Ctr, Zhenjiang, Peoples R China
基金
美国国家科学基金会; 中国博士后科学基金; 中国国家自然科学基金;
关键词
Bombyx mori; dietary restriction; juvenile hormone pathway; transcriptomic; uric acid; LIFE-SPAN; URIC-ACID; PROTEIN-SYNTHESIS; SILKWORM; PATHWAY; HEMOLYMPH; LONGEVITY; STRESS; UREASE; PCR;
D O I
10.1002/arch.21388
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Dietary restriction (DR) refers to a reduction in food intake to induce undernutrition but not malnutrition, which extends the lifespan of multiple species. Although there are invertebrate aging models, such as the Caenorhabditis elegans and Drosophila melanogaster, aging studies in Lepidoptera are few in number and the underlying life-extending molecular mechanisms are not clear. Research on a broader range of animals is necessary to support generalizations on mechanisms of aging and rates of aging. The aim of this study was to further investigate genes and pathways associated with DR in Bombyx mori. Here, we used mRNA deep sequencing (RNA-seq) to further investigate genes and pathways associated with DR. The transcriptome profiles showed that most of the differentially expressed genes were upregulated following DR, and genes involved in amino acid and protein metabolism, RNA metabolism and translation, energy metabolism, nitrogen metabolism, and juvenile hormone pathway-related proteins were particularly affected. DR also affects the metabolism of uric acid and urea, which accumulated in silkworm following DR. We speculate that this may not be due to activation of uric acid biosynthesis, but rather by downregulating the degradation of uric acid and urea. These results may help us to understand the mechanisms by which DR prolong lifespan in insects and other animals.
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页数:13
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