Effect of rosmarinic acid on differentiation and mineralization of MC3T3-E1 osteoblastic cells on titanium surface

被引：15

作者：

Jeong, Moon-Jin ^{[1
]}

Lim, Do-Seon ^{[2
]}

Kim, Sung Ok ^{[3
]}

Park, Cheol ^{[4
]}

Choi, Yung Hyun ^{[5
]}

Jeong, Soon-Jeong ^{[6
,7
]}

机构：

[1] Chosun Univ, Dept Oral Histol & Dev Biol, Sch Dent, Gwangju, South Korea

[2] Eulji Univ, Grad Sch Publ Hlth Sci, Dept Dent Hyg, Seongnam, South Korea

[3] Kyungsung Univ, Dept Food Sci & Biotechnol, Coll Engn, Busan, South Korea

[4] Dong Eui Univ, Div Basic Sci, Coll Liberal Studies, Busan, South Korea

[5] Dong Eui Univ, Coll Korean Med, Dept Biochem, Yangjung Ro 52-57, Busan 47227, South Korea

[6] Youngsan Univ, Coll Hlth Sci, Dept Dent Hyg, Junam Ro 288, Yangsan 50510, Gyeongnam, South Korea

[7] Youngsan Univ, Inst Basic Sci Well Aging, Yangsan, South Korea

来源：

ANIMAL CELLS AND SYSTEMS | 2021年 / 25卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Differentiation; mineralization; osseointegration; rosmarinic acid; titanium;

D O I：

10.1080/19768354.2021.1886987

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Titanium (Ti) is a widely used biomaterial for dental implants because of its outstanding biocompatibility for hard tissues. Osseointegration, the interaction between implanted biomaterials and living cells in bone, is essential for successful implantation. Rosmarinic acid (RA) is a plant-derived phytochemical with low toxicity and side effects and has various effects that can be applied as a therapeutic substance. The MC3T3-E1 osteoblastic cells on the Ti surface in medium with or without 14 mu g/ml RA were used to test RA effects on osteoblast differentiation, cell viability and mineralization during differentiation. RA treatment increased osteoblast differentiation, cell viability and mineralization in MC3T3-E1 osteoblastic cells on Ti surface during differentiation, upregulating Runx-2 and OPG, but downregulating RANKL. This study suggest that RA should be applied as an effective functional and therapeutic substance to enhance osseointegration of osteoblast cells by increasing differentiation, mineralization, and bone formation through the RANKL/RANK/OPG pathway during the differentiation in MC3T3-E1 osteoblastic cells on the Ti surface.

引用

页码：46 / 55

页数：10

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