VCP/p97 Is a Proviral Host Factor for Replication of Chikungunya Virus and Other Alphaviruses

被引：21

作者：

Carissimo, Guillaume ^{[1
]}

Chan, Yi-Hao ^{[1
,2
]}

Utt, Age ^{[3
]}

Chua, Tze-Kwang ^{[1
]}

Abu Bakar, Farhana ^{[1
,4
]}

Merits, Andres ^{[3
]}

Ng, Lisa F. P. ^{[1
,5
,6
]}

机构：

[1] ASTAR, Singapore Immunol Network, Singapore, Singapore

[2] Natl Univ Singapore, NUS Grad Sch Integrat Sci & Engn, Singapore, Singapore

[3] Univ Tartu, Fac Sci & Technol, Inst Technol, Tartu, Estonia

[4] Nanyang Technol Univ, Coll Sci, Sch Biol Sci, Singapore, Singapore

[5] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Biochem, Singapore, Singapore

[6] Univ Liverpool, Inst Infect & Global Hlth, Liverpool, Merseyside, England

来源：

FRONTIERS IN MICROBIOLOGY | 2019年 / 10卷

关键词：

VCP; VCP/p97; alphavirus; chikungunya; host factor; proviral factor; CONTAINING PROTEIN VCP/P97; SINDBIS VIRUS; AAA-ATPASE; PROTEASOME; INHIBITORS; INTERPLAY; CANCER; P97; ER;

D O I：

10.3389/fmicb.2019.02236

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The evolutionarily conserved AAA+ ATPase valosin-containing protein (VCP) was previously shown to be a proviral host factor for several viruses from different viral families such as Flaviviridae, Picornaviridae, and Herpesviridae. VCP was shown to affect trafficking of Sindbis virus receptor and functions as a component of Semliki Forest virus (SFV) replicase compartment. However, the role of this cellular protein was not evaluated during replication of alphaviruses including chikungunya virus (CHIKV). Using siRNA, chemical inhibitors, and trans-replication assays, we show here that VCP is a proviral factor involved in the replication of CHIKV. Immunofluorescence assays confirmed that VCP co-localized with non-structural replicase proteins but not with dsRNA foci possibly due to VCP epitope unavailability. VCP pro-viral role is also observed with other alphaviruses such as o'nyong'nyong virus (ONNV) and SFV in different human cell lines. VCP proviral roles on several viral families now extend to replication of alphaviruses CHIKV and ONNV, emphasizing the pivotal role of VCP in virus-host interaction biology.

引用

页数：15

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