Synergistic antibacterial effects of reuterin and catechin against Streptococcus mutans

被引：18
作者：
Zhang, Guofang [1 ]
Tan, Ying [1 ]
Yu, Tianshu [1 ]
Wang, Sitong [1 ]
Liu, Libo [1 ]
Li, Chun [1 ]
机构：
[1] Northeast Agr Univ, Coll Food Sci, Key Lab Dairy Sci, Harbin 150030, Peoples R China
来源：
LWT-FOOD SCIENCE AND TECHNOLOGY | 2021年 / 139卷
基金：
黑龙江省自然科学基金;
关键词：
Reuterin; Catechin; Streptococcus mutans; Biofilm; Antibacterial effects; LACTOBACILLUS-REUTERI; EPIGALLOCATECHIN GALLATE; BIOFILM FORMATION; 3-HYDROXYPROPIONALDEHYDE; INHIBITION; PATHOGENS; MATRIX; ACID;
D O I：
10.1016/j.lwt.2020.110527
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Reuterin is a broad-spectrum antimicrobial agent widely used to control pathogenic bacteria. Antimicrobial properties of catechin have been well described. However, no information is available regarding their combination effects. The aim of the present study is to evaluate the antibacterial effects of reuterin and catechin alone or in combination against Streptococcus mutans (S. mutans). We found a clear synergistic effect of reuterin in combination with catechin on inhibiting growth of S. mutans. Combined treatment with reuterin and catechin resulted in reduced biofilm biomass and water-insoluble glucan content, and visibly damaged the biofilm structure. Moreover, the expression of biofilm-associated genes was significantly down-regulated after exposure to the combination. Our results demonstrate that the combination of reuterin and catechin exhibits synergistic antimicrobial activity against S. mutans by repressing growth, biofilm formation, water-insoluble glucan production, and virulence genes expression. These findings support this novel combination can be used as a natural antibacterial agent for the prevention of oral diseases.
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页数：7
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