SIGNATURE: A single-particle selection system for molecular electron microscopy

被引：124
作者：
Chen, James Z. [1 ]
Grigorieff, Nikolaus [1 ]
机构：
[1] Brandeis Univ, Dept Biochem, Rosenstiel Basic Med Sci Res Ctr, Howard Hughes Med Inst,WM Keck Inst Cellular Visu, Waltham, MA 02454 USA
来源：
JOURNAL OF STRUCTURAL BIOLOGY | 2007年 / 157卷 / 01期
关键词：
single-particle electron microscopy; automatic particle selection;
D O I：
10.1016/j.jsb.2006.06.001
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
SIGNATURE is a particle selection system for molecular electron microscopy. It applies a hierarchical screening procedure to identify molecular particles in EM micrographs. The user interface of the program provides versatile functions to facilitate image data visualization, particle annotation and particle quality inspection. The system design emphasizes both functionality and usability. This software has been released to the EM community and has been successfully applied to macromolecular structural analyses. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码：168 / 173
页数：6
相关论文
共 7 条
[1]   SIGNAL-TO-NOISE RATIO OF ELECTRON MICROGRAPHS OBTAINED BY CROSS-CORRELATION [J].
FRANK, J ;
ALALI, L .
NATURE, 1975, 256 (5516) :376-379
[2]   Electron cryomicroscopy structure of N-ethyl maleimide sensitive factor at 11 Å resolution [J].
Furst, J ;
Sutton, RB ;
Chen, J ;
Brunger, AT ;
Grigorieff, N .
EMBO JOURNAL, 2003, 22 (17) :4365-4374
[3]   IMAGE-CONTRAST IN HIGH-RESOLUTION ELECTRON-MICROSCOPY OF BIOLOGICAL MACROMOLECULES - TMV IN ICE [J].
HENDERSON, R .
ULTRAMICROSCOPY, 1992, 46 (1-4) :1-18
[4]   Review: Automatic particle detection in electron microscopy [J].
Nicholson, WV ;
Glaeser, RM .
JOURNAL OF STRUCTURAL BIOLOGY, 2001, 133 (2-3) :90-101
[5]   Particle finding in electron micrographs using a fast local correlation algorithm [J].
Roseman, AM .
ULTRAMICROSCOPY, 2003, 94 (3-4) :225-236
[6]   Automatic particle selection: results of a comparative study [J].
Zhu, YX ;
Carragher, B ;
Glaeser, RM ;
Fellmann, D ;
Bajaj, C ;
Bern, M ;
Mouche, F ;
de Haas, F ;
Hall, RJ ;
Kriegman, DJ ;
Ludtke, SJ ;
Mallick, SP ;
Penczek, PA ;
Roseman, AM ;
Sigworth, FJ ;
Volkmann, N ;
Potter, CS .
JOURNAL OF STRUCTURAL BIOLOGY, 2004, 145 (1-2) :3-14
[7]   Automatic particle detection through efficient Hough transforms [J].
Zhu, YX ;
Carragher, B ;
Mouche, F ;
Potter, CS .
IEEE TRANSACTIONS ON MEDICAL IMAGING, 2003, 22 (09) :1053-1062
1