Enhancement of protein expression in insect cells by a lobster tropomyosin cDNA leader sequence

被引：69

作者：

Sano, KI ^{[1
]}

Maeda, K ^{[1
]}

Oki, M ^{[1
]}

Maéda, Y ^{[1
]}

机构：

[1] RIKEN, Harima Inst, SPring 8, Lab Struct Biochem, Sayo, Hyogo 6795148, Japan

来源：

FEBS LETTERS | 2002年 / 532卷 / 1-2期

关键词：

baculovirus; Sf9; recombinant protein expression; leader sequence; tropomyosin;

D O I：

10.1016/S0014-5793(02)03659-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a cis element that dramatically increases the expression levels of exogenous genes in baculovirus-infected insect cells. This 21 bp sequence element is derived from a 5' untranslated leader sequence of a lobster tropomyosin cDNA (L21). By using a transfer vector carrying L21, the expression levels of tropomyosin and luciferase were 20- and seven-fold higher with L21 than without L21, respectively. L21 has both the Kozak sequence and the A-rich sequence found in the polyhedrin leader sequence. We assume that both sequence elements are essential for the enhancement of protein expression in the baculovirus-based expression system. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：143 / 146

页数：4

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