A flow-through microfluidic system for the detection of circulating melanoma cells

被引：32

作者：

Prathap, Mylamparambil Udayan Anu ^{[1
]}

Castro-Perez, Edgardo ^{[2
]}

Jimenez-Torres, Jose A. ^{[3
]}

Setaluri, Vijaysaradhi ^{[2
]}

Gunasekaran, Sundaram ^{[1
]}

机构：

[1] Univ Wisconsin Madison, Dept Biol Syst Engn, 460 Henry Mall, Madison, WI 53706 USA

[2] Univ Wisconsin Madison, Sch Med & Publ Hlth, Dept Dermatol, 1300 Univ Ave, Madison, WI 53706 USA

[3] Univ Wisconsin Madison, Coll Engn, Microtechnol Med & Biol Lab Biomed Engn, Madison, WI 53705 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2019年 / 142卷

基金：

美国国家卫生研究院;

关键词：

Melanoma cells; Electrochemical immunosensor; Microfluidics; Polyaniline nanofibers; POLYMERASE-CHAIN-REACTION; TUMOR-CELLS; ELECTROCHEMICAL IMMUNOSENSOR; SENSITIVE DETECTION; PERIPHERAL-BLOOD; IMMUNOASSAY; STAGE; ASSOCIATION; BIOMARKER; UTILITY;

D O I：

10.1016/j.bios.2019.111522

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We report the fabrication of polyaniline nanofiber (PANI)-modified screen-printed electrode (PANI/SPE) incorporated in a poly-dimethylsiloxane (PDMS) microfluidic channel for the detection of circulating tumor cells. We employed this device to detect melanoma skin cancer cells through specific immunogenic binding of cell surface biomarker melanocortin 1 receptor (MC1R) to anti-MC1R antibody. The antibody-functionalized PANI/SPE was used in batch-continuous flow-through fashion. An aqueous cell suspension of ferri/ferrocyanide at a flow rate of 1.5 mL/min was passed over the immunosensor, which allowed for continuous electrochemical measurements. The sensor performed exceptionally well affording an ultralow limit of quantification of 1 melanoma cell/mL, both in buffer and when mixed with peripheral blood mononuclear cells, and the response was log-linear over the range of 10-9000 melanoma cells/10 mL.

引用

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