A novel class of cationic gemini surfactants showing efficient in vitro gene transfection properties

被引：72

作者：

Camilleri, P

Kremer, A

Edwards, AJ

Jennings, KH

Jenkins, O

Marshall, I

McGregor, C

Neville, W

Rice, SQ

Smith, RJ

Wilkinson, MJ

Kirby, AJ

机构：

[1] SmithKline Beecham Pharmaceut, Harlow CM19 5AW, Essex, England

[2] Univ Cambridge, Chem Lab, Cambridge CB2 1EW, England

来源：

CHEMICAL COMMUNICATIONS | 2000年 / 14期

关键词：

D O I：

10.1039/b001742f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Five novel peptide-based cationic gemini surfactants have been synthesised and their ability to transfect plasmid DNA containing the luciferase gene has been examined. Three of these detergents, differing in the number of positive charges per molecule at neutral pH, mediated transfection on their own. However, their efficiency increased markedly on the addition of a neutral colipid and a basic polypeptide.

引用

页码：1253 / 1254

页数：2

共 7 条

[1]

FELGNER JH, 1994, J BIOL CHEM, V269, P2550

[2] LIPOFECTION - A HIGHLY EFFICIENT, LIPID-MEDIATED DNA-TRANSFECTION PROCEDURE [J].