Osteogenic potential of human adipose-tissue-derived mesenchymal stromal cells cultured on 3D-printed porous structured titanium

被引：24

作者：

Lewallen, Eric A. ^{[1
]}

Jones, Dakota L. ^{[1
,2
]}

Dudakovic, Amel ^{[1
]}

Thaler, Roman ^{[1
]}

Paradise, Christopher R. ^{[1
]}

Kremers, Hilal M. ^{[3
]}

Abdel, Matthew P. ^{[1
]}

Kakar, Sanjeev ^{[1
]}

Dietz, Allan B. ^{[4
]}

Cohen, Robert C. ^{[5
]}

Lewallen, David G. ^{[1
]}

van Wijnen, Andre J. ^{[1
,2
,6
]}

机构：

[1] Mayo Clin, Dept Orthoped Surg, 200 First St SW, Rochester, MN 55905 USA

[2] Mayo Clin, Dept Biomed Engn & Physiol, Mayo Grad Sch, 200 First St SW, Rochester, MN 55905 USA

[3] Mayo Clin, Dept Hlth Sci Res, Coll Med, 200 First St SW, Rochester, MN 55905 USA

[4] Mayo Clin, Dept Lab Med & Pathol, 200 First St SW, Rochester, MN 55905 USA

[5] Stryker Orthoped, 325 Corp Dr, Mahwah, NJ 07430 USA

[6] Mayo Clin, Dept Biochem & Mol Biol, 200 First St SW, Rochester, MN 55905 USA

来源：

GENE | 2016年 / 581卷 / 02期

关键词：

Mesenchymal stem cell; Biomaterial; Gene expression; Extracellular matrix; Scanning electron microscopy; HUMAN OSTEOBLASTS; STEM-CELLS; TANTALUM; DIFFERENTIATION; PROLIFERATION; HIP; ARTHROPLASTY; VALIDATION; SCAFFOLDS; SURFACES;

D O I：

10.1016/j.gene.2016.01.015

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Integration of porous metal prosthetics, which restore form and function of irreversibly damaged joints, into remaining healthy bone is critical for implant success. We investigated the biological properties of adipose tissue-derived mesenchymal stromal/stem cells (AMSCs) and addressed their potential to alter the in vitro microenvironment of implants. We employed human AMSCs as a practical source for musculoskeletal applications because these cells can be obtained in large quantities, are multipotent, and have trophic paracrine functions. AMSCs were cultured on surgical-grade porous titanium disks as a model for orthopedic implants. We monitored cell/substrate attachment, cell proliferation, multipotency, and differentiation phenotypes of AMSCs upon osteogenic induction. High-resolution scanning electron microscopy and histology revealed that AMSCs adhere to the porous metallic surface. Compared to standard tissue culture plastic, AMSCs grown in the porous titanium micro environment showed differences in temporal expression for genes involved in cell cycle progression (CCNB2, HIST2H4), extracellular matrix production (COL1A1, COL3A1), mesenchymal lineage identity (ACTA2, CD248, CD44), osteoblastic transcription factors (DLX3, DLX5,1D3), and epigenetic regulators (EZH1, EZH2). We conclude that metal orthopedic implants can be effectively seeded with clinical-grade stem/stromal cells to create a preconditioned implant. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：95 / 106

页数：12

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