Monocyte chemotactic protein-1 concentration in peritoneal fluid of women with endometriosis and its modulation of expression in mesothelial cells

被引:161
作者
Arici, A
Oral, E
Attar, E
Tazuke, SI
Olive, DL
机构
[1] Div. of Reproductive Endocrinology, Dept. of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT
[2] Dept. of Obstetrics and Gynecology, Yale University School of Medicine, 333 Cedar Street, New Haven
关键词
endometriosis; monocyte chemotactic protein-1; peritoneal fluid; cytokines;
D O I
10.1016/S0015-0282(97)81440-9
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To investigate monocyte chemotactic protein-1 concentrations in the peritoneal fluid (PF) of women with or without endometriosis, then assess peritoneal mesothelial cells as a potential source of monocyte chemotactic protein-1. Design: Prospective study. Setting: University medical center. Patient(s): Women with (n = 60) or without (n = 18) endometriosis. Intervention(s): First monocyte chemotactic protein-1 levels in PF were measured, then mesothelial cells in culture were treated with cytokines. Main Outcome Measure(s): In PF and culture supernatants, monocyte chemotactic protein-1 was measured by ELISA. In vitro monocyte chemotactic protein-1 messenger RNA expression was evaluated by Northern analysis. Result(s): The median concentration of monocyte chemotactic protein-1 in PF of control women was 137 pg/mL (conversion factor to SI unit, 0.115; range, 12 to 418 pg/mL); that of women with moderate endometriosis was 205 pg/mL (range 65 to 6,000 pg/mL; and that of those with severe endometriosis was 1,165 pg/mL (0 to 2,602 pg/mL). Within the moderate to severe endometriosis group, monocyte chemotactic protein-1 levels were higher in women with untreated endometriosis (354 pg/mL range 0 to 6,000 pg/mL) than in women receiving GnRH agonist (128 pg/mL, range 0 to 216 pg/mL). In the control group, monocyte chemotactic protein-1 levels were higher in the proliferative phase than in the secretory phase. Mesothelial cells produced constitutively monocyte chemotactic protein-1; moreover, both interleukin-l alpha and tumor necrosis factor-alpha induced higher levels of monocyte chemotactic protein-1. Conclusion(s): Levels of monocyte chemotactic protein-1 in PF were higher during the proliferative phase than secretory phase of control women and increased in moderate to severe endometriosis. The regulated expression of monocyte chemotactic protein-1 may recruit macrophages into PF and contribute to the pathogenesis of endometriosis. (C) 1997 by American Society for Reproductive Medicine.
引用
收藏
页码:1065 / 1072
页数:8
相关论文
共 25 条
[11]   THE EFFECT OF GROWTH-FACTORS ON THE PROLIFERATION OF HUMAN ENDOMETRIAL STROMAL CELLS IN CULTURE [J].
HAMMOND, MG ;
OH, ST ;
ANNERS, J ;
SURREY, ES ;
HALME, J .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1993, 168 (04) :1131-1138
[12]  
HANEY AF, 1981, FERTIL STERIL, V35, P696
[13]   REDUCTION OF THE INTRAPERITONEAL INFLAMMATION ASSOCIATED WITH ENDOMETRIOSIS BY TREATMENT WITH MEDROXYPROGESTERONE ACETATE [J].
HANEY, AF ;
WEINBERG, JB .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1988, 159 (02) :450-454
[14]  
LAROCCA PJ, 1984, CANCER RES, V44, P2991
[15]   INCREASED CHEMOTACTIC ACTIVITY OF PERITONEAL-FLUID IN PATIENTS WITH ENDOMETRIOSIS [J].
LEIVA, MC ;
HASTY, LA ;
PFEIFER, S ;
MASTROIANNI, L ;
LYTTLE, CR .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1993, 168 (02) :592-598
[16]   PREVALENCE AND GENESIS OF ENDOMETRIOSIS [J].
MAHMOOD, TA ;
TEMPLETON, A .
HUMAN REPRODUCTION, 1991, 6 (04) :544-549
[17]   AGE AND PERITONEAL FLUID CELLULAR DISTRIBUTION IN WOMEN 20-40 YEARS OF AGE [J].
MCGOWAN, L ;
PITKOW, HS ;
DAVIS, RH .
EXPERIENTIA, 1976, 32 (03) :314-315
[18]  
OLIVE DL, 1987, OBSTET GYNECOL, V69, P412
[19]  
OLIVE DL, 1991, AM J OBSTET GYNECOL, V164, P953
[20]  
ORAL DL, 1996, AM J OBSTET GYNECOL, V14, P257