Biosensor for Hydrogen Peroxide Based on Chitosan and Nanoparticle Complex Film-Modified Glassy-Carbon Electrodes

被引：10

作者：

Xu, Qiao ^{[1
]}

Wang, Jian-Hao ^{[1
]}

Wang, Zhan ^{[1
]}

Wang, Hai-Qiao ^{[1
]}

Yang, Qin ^{[1
]}

Zhao, Yuan-Di ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Hubei Bioinformat & Mol Imaging Key Lab, Wuhan Natl Lab Optoelect, Wuhan 430074, Hubei, Peoples R China

来源：

ANALYTICAL LETTERS | 2009年 / 42卷 / 15期

基金：

中国国家自然科学基金;

关键词：

Chitosan; electrochemistry; hemoglobin; water-soluble CdTe nanoparticle; CDTE QUANTUM DOTS; DIRECT ELECTROCHEMISTRY; HEME-PROTEINS; HEMOGLOBIN; ELECTROCATALYSIS; SYSTEMS;

D O I：

10.1080/00032710903082739

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A biosensor for hydrogen peroxide was fabricated by co-immobilizing cadmium telluride (CdTe) nanoparticles, chitosan, and hemoglobin (Hb) matrix. There was a pair of nearly reversible redox peaks around -0.360V, and the electrochemical behavior of Hb was a surface-controlled process, with an electron-transfer rate constant of 1.36s-1 and surface coverage of 2.62x10-10mol cm-2. Fourier transform infrared (FT-IR) spectra and ultraviolet-visible (UV-vis) spectra indicated that Hb sustained its natural conformation. It was demonstrated that Hb in the matrix kept its bioactivity and exhibited catalytic ability toward H2O2, with a response ranging from 7.44x10-6 to 6.95x10-4M and a detection limit of 2.23x10-6M.

引用

页码：2496 / 2508

页数：13

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