Biosensor for Hydrogen Peroxide Based on Chitosan and Nanoparticle Complex Film-Modified Glassy-Carbon Electrodes

被引：10

作者：

Xu, Qiao ^{[1
]}

Wang, Jian-Hao ^{[1
]}

Wang, Zhan ^{[1
]}

Wang, Hai-Qiao ^{[1
]}

Yang, Qin ^{[1
]}

Zhao, Yuan-Di ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Hubei Bioinformat & Mol Imaging Key Lab, Wuhan Natl Lab Optoelect, Wuhan 430074, Hubei, Peoples R China

来源：

ANALYTICAL LETTERS | 2009年 / 42卷 / 15期

基金：

中国国家自然科学基金;

关键词：

Chitosan; electrochemistry; hemoglobin; water-soluble CdTe nanoparticle; CDTE QUANTUM DOTS; DIRECT ELECTROCHEMISTRY; HEME-PROTEINS; HEMOGLOBIN; ELECTROCATALYSIS; SYSTEMS;

D O I：

10.1080/00032710903082739

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A biosensor for hydrogen peroxide was fabricated by co-immobilizing cadmium telluride (CdTe) nanoparticles, chitosan, and hemoglobin (Hb) matrix. There was a pair of nearly reversible redox peaks around -0.360V, and the electrochemical behavior of Hb was a surface-controlled process, with an electron-transfer rate constant of 1.36s-1 and surface coverage of 2.62x10-10mol cm-2. Fourier transform infrared (FT-IR) spectra and ultraviolet-visible (UV-vis) spectra indicated that Hb sustained its natural conformation. It was demonstrated that Hb in the matrix kept its bioactivity and exhibited catalytic ability toward H2O2, with a response ranging from 7.44x10-6 to 6.95x10-4M and a detection limit of 2.23x10-6M.

引用

页码：2496 / 2508

页数：13

共 50 条

[31] A Hydrogen Peroxide Biosensor Based on Room Temperature Ionic Liquid Functionalized Graphene Modified Carbon Ceramic Electrode
He, Yaping
Zheng, Jianbin
Li, Kuangtian
Sheng, Qinglin
Qiao, Ningqiang
CHINESE JOURNAL OF CHEMISTRY, 2010, 28 (12) : 2507 - 2512
[32] A Novel Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Immobilized on Poly(aniline-co-o-aminobenzoic acid) Modified Glassy Carbon Electrode Coated with Chitosan Film
Chairam, Sanoe
Buddhalee, Peerawich
Amatatongchai, Maliwan
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE, 2013, 8 (08): : 10250 - 10264
[33] A sensitive amperometric hydrogen peroxide sensor based on thionin/EDTA/carbon nanotubes-chitosan composite film modified electrode
Zhang, Keying
Zhang, Li
Xu, Jigui
Wang, Cong
Geng, Tao
Wang, Hongyan
Zhu, Jun
MICROCHIMICA ACTA, 2010, 171 (1-2) : 139 - 144
[34] A hydrogen peroxide biosensor with high stability based on gelatin-multiwalled carbon nanotubes modified glassy carbon electrode
Yulin Wang
Tianyu Li
Weijie Zhang
Yaqin Huang
Journal of Solid State Electrochemistry, 2014, 18 : 1981 - 1987
[35] Hydrogen peroxide biosensor based on myoglobin/colloidal gold nanoparticles immobilized on glassy carbon electrode by a Nafion film
Yang, WW
Li, YC
Bai, Y
Sun, CQ
SENSORS AND ACTUATORS B-CHEMICAL, 2006, 115 (01) : 42 - 48
[36] Electrochemical detection of carbohydrates at carbon-nanotube modified glassy-carbon electrodes
Deo, RP
Wang, J
ELECTROCHEMISTRY COMMUNICATIONS, 2004, 6 (03) : 284 - 287
[37] Modified carbon nanoparticle-chitosan film electrodes:: Physisorption versus chemisorption
Rassaei, Liza
Sillanpaa, Mika
Marken, Frank
ELECTROCHIMICA ACTA, 2008, 53 (19) : 5732 - 5738
[38] Biomimetic behavior and nanomolar detection of hydrogen peroxide on an electrochemically pre-treated hematin modified glassy carbon electrode
Buoro, Rafael M.
Bacil, Raphael P.
Sanz, Caroline G.
Campos, Othon S.
Serrano, Silvia H. P.
SENSORS AND ACTUATORS B-CHEMICAL, 2017, 250 : 169 - 178
[39] Immobilization of Hemoglobin on the Gold Colloid Modified Pretreated Glassy Carbon Electrode for Preparing a Novel Hydrogen Peroxide Biosensor
Yang Liu
Qi-Yu Jiang
Shuang-Yun Lu
Ye Zhang
Hai-Ying Gu
Applied Biochemistry and Biotechnology, 2009, 152 : 418 - 427
[40] Hydrogen peroxide biosensor based on gold nanoparticles/thionine/gold nanoparticles/multi-walled carbon nanotubes-chitosans composite film-modified electrode
Li, Shenfeng
Zhu, Xiaoying
Zhang, Wei
Xie, Guoming
Feng, Wenli
APPLIED SURFACE SCIENCE, 2012, 258 (07) : 2802 - 2807

← 1 2 3 4 5 →