Biosensor for Hydrogen Peroxide Based on Chitosan and Nanoparticle Complex Film-Modified Glassy-Carbon Electrodes

被引：10
|
作者：
Xu, Qiao [1 ]
Wang, Jian-Hao [1 ]
Wang, Zhan [1 ]
Wang, Hai-Qiao [1 ]
Yang, Qin [1 ]
Zhao, Yuan-Di [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Hubei Bioinformat & Mol Imaging Key Lab, Wuhan Natl Lab Optoelect, Wuhan 430074, Hubei, Peoples R China
来源：
ANALYTICAL LETTERS | 2009年 / 42卷 / 15期
基金：
中国国家自然科学基金;
关键词：
Chitosan; electrochemistry; hemoglobin; water-soluble CdTe nanoparticle; CDTE QUANTUM DOTS; DIRECT ELECTROCHEMISTRY; HEME-PROTEINS; HEMOGLOBIN; ELECTROCATALYSIS; SYSTEMS;
D O I：
10.1080/00032710903082739
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A biosensor for hydrogen peroxide was fabricated by co-immobilizing cadmium telluride (CdTe) nanoparticles, chitosan, and hemoglobin (Hb) matrix. There was a pair of nearly reversible redox peaks around -0.360V, and the electrochemical behavior of Hb was a surface-controlled process, with an electron-transfer rate constant of 1.36s-1 and surface coverage of 2.62x10-10mol cm-2. Fourier transform infrared (FT-IR) spectra and ultraviolet-visible (UV-vis) spectra indicated that Hb sustained its natural conformation. It was demonstrated that Hb in the matrix kept its bioactivity and exhibited catalytic ability toward H2O2, with a response ranging from 7.44x10-6 to 6.95x10-4M and a detection limit of 2.23x10-6M.
引用
收藏
页码：2496 / 2508
页数：13
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