Characterization of sugars from model and enzyme-mediated pulp hydrolyzates using high-performance liquid chromatography coupled to evaporative light scattering detection

被引：26

作者：

Filson, Paul B. ^{[1
]}

Dawson-Andoh, Benjamin E. ^{[1
]}

机构：

[1] W Virginia Univ, Davis Coll Agr Forestry & Consumer Sci, Div Forestry & Nat Resources, Morgantown, WV 26506 USA

来源：

BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 24期

关键词：

Recycled pulp; Hardwood and pine dissolving pulps; Sugars; Endoglucanase; Hydrolyzates; MICROCRYSTALLINE CELLULOSE; NANOCRYSTALS;

D O I：

10.1016/j.biortech.2008.12.067

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

High-performance liquid chromatography (HPLC) coupled to an evaporative light scattering detector was used to quantitatively determine glucose and cellobiose in hydrolyzates from the production of cellulose nanofillers from modified lignocellulosic materials. Prevail Carbohydrate ES 5 mu column proved more suitable for achieving the chromatographic separation of the model pulp hydrolyzate into its constituent sugars than the YMC-Pack Polyamine column. Linear calibration curves for the various sugars in the mixtures were developed. Glucose and cellobiose were clearly detectable in pulp hydrolyzates obtained from enzyme-mediated hydrolysis of recycled pulp, pine and hardwood dissolving pulps. Finally, the amount of glucose in the pulp hydrolyzates was generally higher than cellobiose. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：6661 / 6664

页数：4

共 23 条

[1] GAS-CHROMATOGRAPHIC DETERMINATION OF HYDROLYZED SUGARS IN COMMERCIAL GUMS [J].