Resources and Methods for Engineering "Designer" Glycan-Binding Proteins

被引：9

作者：

Warkentin, Ruben ^{[1
,2
,3
]}

Kwan, David H. ^{[1
,2
,3
,4
]}

机构：

[1] Concordia Univ, Dept Biol, Ctr Appl Synthet Biol, 7141 Sherbrooke St West, Montreal, PQ H4B 1R6, Canada

[2] Concordia Univ, Ctr Struct & Funct Genom, 7141 Sherbrooke St West, Montreal, PQ H4B 1R6, Canada

[3] Quebec Network Res Prot Funct Struct & Engn, PROTEO, Quebec City, PQ G1V 0A6, Canada

[4] Concordia Univ, Dept Chem & Biochem, 7141 Sherbrooke St West, Montreal, PQ H4B 1R6, Canada

来源：

MOLECULES | 2021年 / 26卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

glycobiology; glycan-binding protein; lectins; protein engineering; glycans; carbohydrates; directed evolution; glycan immobilization; SURFACE-PLASMON RESONANCE; CARBOHYDRATE-BINDING; ESCHERICHIA-COLI; HIGH-AFFINITY; ACTIVE-SITE; IN-VITRO; SUBSTRATE-SPECIFICITY; PHAGE DISPLAY; ACID-BINDING; SIALIC-ACID;

D O I：

10.3390/molecules26020380

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This review provides information on available methods for engineering glycan-binding proteins (GBP). Glycans are involved in a variety of physiological functions and are found in all domains of life and viruses. Due to their wide range of functions, GBPs have been developed with diagnostic, therapeutic, and biotechnological applications. The development of GBPs has traditionally been hindered by a lack of available glycan targets and sensitive and selective protein scaffolds; however, recent advances in glycobiology have largely overcome these challenges. Here we provide information on how to approach the design of novel "designer" GBPs, starting from the protein scaffold to the mutagenesis methods, selection, and characterization of the GBPs.

引用

页数：25

共 121 条

[1] Engineering of PA-IIL lectin from Pseudomonas aeruginosa -: Unravelling the role of the specificity loop for sugar preference [J].