Cross-Interaction Chromatography: A Rapid Method to Identify Highly Soluble Monoclonal Antibody Candidates

被引：78

作者：

Jacobs, Steven A. ^{[1
]}

Wu, Sheng-Jiun ^{[1
]}

Feng, Yiqing ^{[1
]}

Bethea, Deidra ^{[1
]}

O'Neil, Karyn T. ^{[1
]}

机构：

[1] Centocor R&D, Discovery Res, Radnor, PA 19087 USA

来源：

PHARMACEUTICAL RESEARCH | 2010年 / 27卷 / 01期

关键词：

aggregation; cross-interaction chromatography; monoclonal antibody; protein solubility; SELF-INTERACTION CHROMATOGRAPHY; MULTIPLEXED FLOW-CYTOMETRY; SINGLE-CHAIN ANTIBODIES; PROTEIN SOLUBILITY; STABILITY; COEFFICIENTS; MECHANISM; LIBRARIES; AFFINITY; DISPLAY;

D O I：

10.1007/s11095-009-0007-z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Purpose. To develop a high-throughput cross-interaction chromatography screening method to rapidly identify antibody candidates with poor solubility using microgram quantities of purified material. Methods. A specific recombinant antibody or bulk polyclonal IgG purified from human serum was chemically coupled to an NHS-activated chromatography resin. The retention times of numerous monoclonal antibodies were determined on this resin using an HPLC and compared to the solubility of each antibody estimated by ultrafiltration. Results. Retention times of the antibodies tested were found to be inversely related to solubility, with antibodies prone to precipitate at low concentrations in PBS being retained longer on the columns with broader peaks. The technique was successfully used to screen microgram quantities of a panel of therapeutic antibodies to identify candidates with low solubility in PBS. Conclusions. The cross-interaction chromatography methods described can be used to screen large panels of recombinant antibodies in order to discover those with low solubility. Addition of this tool to the array of tools available for characterization of affinity and activity of antibody therapeutic candidates will improve selection of candidates with biophysical properties favorable to development of high concentration antibody formulations.

引用

页码：65 / 71

页数：7

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