Involvement of the Calcium-sensing Receptor in Human Taste Perception

被引:239
作者
Ohsu, Takeaki [2 ]
Amino, Yusuke [3 ]
Nagasaki, Hiroaki [4 ]
Yamanaka, Tomohiko [4 ]
Takeshita, Sen [2 ]
Hatanaka, Toshihiro [2 ]
Maruyama, Yutaka
Miyamura, Naohiro [4 ]
Eto, Yuzuru [1 ,2 ]
机构
[1] Ajinomoto Co Inc, Inst Life Sci, Kawasaki Ku, Kawasaki, Kanagawa 2108681, Japan
[2] Pharmaceut Res Labs, Kawasaki, Kanagawa 2108681, Japan
[3] AminoSci Labs, Kawasaki, Kanagawa 2108681, Japan
[4] Food Prod Co, Kawasaki, Kanagawa 2108681, Japan
关键词
EXTRACELLULAR CA2+-SENSING RECEPTOR; PROTEIN-COUPLED RECEPTORS; GAMMA-GLUTAMYL PEPTIDES; GASTROINTESTINAL-TRACT; GLUTATHIONE; FAMILY; CLONING; ROLES; WATER; RATS;
D O I
10.1074/jbc.M109.029165
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
By human sensory analyses, we found that various extracellular calcium-sensing receptor (CaSR) agonists enhance sweet, salty, and umami tastes, although they have no taste themselves. These characteristics are known as "kokumi taste" and often appear in traditional Japanese cuisine. Although GSH is a typical kokumi taste substance (taste enhancer), its mode of action is poorly understood. Here, we demonstrate how the kokumi taste is enhanced by the CaSR, a close relative of the class C G-protein-coupled receptors T1R1, T1R2, and T1R3 (sweet and umami receptors). We identified a large number of CaSR agonist gamma-glutamyl peptides, including GSH (gamma-Glu-Cys-Gly) and gamma-Glu-Val-Gly, and showed that these peptides elicit the kokumi taste. Further analyses revealed that some known CaSR agonists such as Ca2+, protamine, polylysine, L-histidine, and cinacalcet (a calcium-mimetic drug) also elicit the kokumi taste and that the CaSR-specific antagonist, NPS-2143, significantly suppresses the kokumi taste. This is the first report indicating a distinct function of the CaSR in human taste perception.
引用
收藏
页码:1016 / 1022
页数:7
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