Molecular Characterization of Sulfate-Reducing Bacteria Community in Surface Sediments from the Adjacent Area of Changjiang Estuary

被引:11
作者
Zhang Yu [1 ,2 ]
Zhen Yu [1 ,2 ,5 ]
Mi Tiezhu [1 ,2 ]
He Hui [3 ]
Yu Zhigang [4 ,5 ]
机构
[1] Ocean Univ China, Coll Environm Sci & Engn, Qingdao 266100, Peoples R China
[2] Minist Educ, Key Lab Marine Environm & Ecol, Qingdao 266100, Peoples R China
[3] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
[4] Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Peoples R China
[5] Qingdao Natl Oceanog Lab, Funct Lab Marine Ecol & Environm Sci, Qingdao 266100, Peoples R China
基金
中国国家自然科学基金;
关键词
sulfate-reducing bacteria (SRB); dissimilatory sulfite reductase beta subunit gene (dsrB); diversity; SULFITE REDUCTASE GENES; GUAYMAS BASIN; HYDROTHERMAL SEDIMENTS; PHYLOGENETIC DIVERSITY; MICROBIAL DIVERSITY; MARINE SEDIMENT; HOT-SPRINGS; SALT-MARSH; PROKARYOTES; SEA;
D O I
10.1007/s11802-016-2781-7
中图分类号
P7 [海洋学];
学科分类号
0707 ;
摘要
Sulfate-reducing bacteria (SRB), which obtain energy from dissimilatory sulfate reduction, play a vital role in the carbon and sulfur cycles. The dissimilatory sulfite reductase (Dsr), catalyzing the last step in the sulfate reduction pathway, has been found in all known SRB that have been tested so far. In this study, the diversity of SRB was investigated in the surface sediments from the adjacent area of Changjiang Estuary by PCR amplification, cloning and sequencing of the dissimilatory sulfite reductase beta subunit gene (dsrB). Based on dsrB clone libraries constructed in this study, diversified SRB were found, represented by 173 unique OTUs. Certain cloned sequences were associated with Desulfobacteraceae, Desulfobulbaceae, and a large fraction (60%) of novel sequences that have deeply branched groups in the dsrB tree, indicating that novel SRB inhabit the surface sediments. In addition, correlations of the SRB assemblages with environmental factors were analyzed by the linear model-based redundancy analysis (RDA). The result revealed that temperature, salinity and the content of TOC were most closely correlated with the SRB communities. More information on SRB community was obtained by applying the utility of UniFrac to published dsrB gene sequences from this study and other 9 different kinds of marine environments. The results demonstrated that there were highly similar SRB genotypes in the marine and estuarine sediments, and that geographic positions and environmental factors influenced the SRB community distribution.
引用
收藏
页码:107 / 116
页数:10
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