Adenylate kinase amplification of ATP bioluminescence for hygiene monitoring in the food and beverage industry

被引：1

作者：

Corbitt, AJ

Bennion, N

Forsythe, SJ ^{[1
]}

机构：

[1] Nottingham Trent Univ, Dept Life Sci, Nottingham NG11 8NS, England

[2] Biotrace Ltd, Bridgend, Wales

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2000年 / 30卷 / 06期

关键词：

D O I：

10.1046/j.1472-765x.2000.00744.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fourteen food residues, Escherichia coli O157:H7 and Staphylococcus aureus on stainless steel surfaces were detected using a combined assay with adenylate kinase as a cellular marker and ATP bioluminescence. The limit of sensitivity ranged from 0 . 02 to 708 mu g for minced meat and broccoli, respectively. Both methods gave the same detection limit (10(5) cfu) for E. coli and Staph. aureus on stainless steel surfaces. The combined adenylate kinase-ATP assay is applicable to monitor the hygiene of work surfaces, especially those prone to contamination by meat and vegetable residues.

引用

页码：443 / 447

页数：5