Genetic Depletion of Class I Odorant Receptors Impacts Perception of Carboxylic Acids

被引:13
作者
Cichy, Annika [1 ]
Shah, Ami [1 ]
Dewan, Adam [1 ,2 ]
Kaye, Sarah [1 ]
Bozza, Thomas [1 ]
机构
[1] Northwestern Univ, Dept Neurobiol, 2205 Tech Dr, Evanston, IL 60208 USA
[2] Florida State Univ, Dept Psychol, 1107 W Call St, Tallahassee, FL 32306 USA
关键词
OLFACTORY SENSORY NEURONS; MOUSE; BULB; EXPRESSION; RESPONSES; SENSITIVITY; MOLECULES; SEQUENCE; DELETION; PLATFORM;
D O I
10.1016/j.cub.2019.06.085
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The mammalian main olfactory pathway detects myriad volatile chemicals using >1,000 odorant receptor (OR) genes, which are organized into two phylogenetically distinct classes (class I and class II). An important question is how these evolutionarily conserved classes contribute to odor perception. Here, we report functional inactivation of a large number of class I ORs in mice via identification and deletion of a local cis-acting enhancer in the class I gene cluster. This manipulation reduced expression of half of the 131 intact class I genes. The resulting class I-depleted mice exhibited a significant reduction in the number of glomeruli responding to carboxylic acids-chemicals associated with microbial action and body odors. These mice also exhibit a change in odor perception marked by a selective loss of behavioral aversion to these compounds. Together, our data demonstrate that class I ORs play a critical role in representing a class of biologically relevant chemosignals.
引用
收藏
页码:2687 / +
页数:15
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