Identification of Valeriana fauriei and other Eurasian medicinal valerian by DNA polymorphisms in psbA-trnH intergenic spacer sequences in chloroplast DNA

被引:3
作者
Fujii, Taichi [1 ]
Mori, Takako [1 ]
Tatsuo, Yoshiaki [2 ]
Takao, Yasumasa [2 ]
Fujino, Hiroharu [2 ]
Tsuchida, Takashi [3 ]
Minami, Motoyasu [1 ]
机构
[1] Chubu Univ, Coll Biosci & Biotechnol, 1200 Matsumoto Cho, Kasugai, Aichi 4878501, Japan
[2] Univ Toyama, Sch Pharm & Pharmaceut Sci, Expt Stn Med Plant Res, 2630 Sugitani, Toyama, Toyama 9300194, Japan
[3] Kobayashi Pharmaceut Co Ltd, Cent R&D Lab, 1-30-3 Toyokawa, Ibaraki, Osaka 5670057, Japan
关键词
Valeriana fauriei; Eurasian medicinal valerian; psbA– trnH intergenic spacer; ITS; BIOGEOGRAPHY; PHYLOGENY; PLANTS;
D O I
10.1007/s11418-021-01488-z
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
In order to differentiate among Valeriana fauriei Briq. and other Eurasian medicinal valerian (V. dioica L., V. hardwickii Wall., V. jatamansi Jones, and V. officinalis L.), we attempted to establish DNA markers. DNA sequences for the psbA-trnH intergenic spacer region of chloroplast DNA (psbA-trnH) and 18S ribosomal RNA, internal transcribed spacer 1 (ITS1), 5.8S ribosomal RNA, internal transcribed spacer 2 (ITS2), and 28S ribosomal RNA of nuclear DNA in V. fauriei and other Eurasian medicinal valerian were compared. Using partial sequences of psbA-trnH (nucleotide positions 1-75 from the 5 ' end of the intergenic spacer region), V. fauriei and other Eurasian medicinal valerian could be correctly identified to the species level. In addition, the partial sequences of psbA-trnH in V. fauriei contained five different haplotypes, and it was possible to distinguish the origins of valerian from Japan and Eurasia (China and Korea). On the other hand, individuals had heterogeneous sequences of ITS1 and ITS2, making it impossible to use direct sequencing and DNA markers of ITS1 and ITS2 to distinguish species and origins of V. fauriei and other Eurasian medicinal valerian.
引用
收藏
页码:699 / 706
页数:8
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