Cell wall biogenesis in a double chitin synthase mutant (chsG-/chsE-) of Aspergillus fumigatus

被引:71
作者
Mellado, E
Dubreucq, G
Mol, P
Sarfati, J
Paris, S
Diaquin, M
Holden, DW
Rodriguez-Tudela, JL
Latgé, JP
机构
[1] Inst Salud Carlos III, Ctr Nacl Microbiol, Unidad Micol, Madrid 28220, Spain
[2] Inst Pasteur, Unite Aspergillus, F-75015 Paris, France
[3] Univ London Imperial Coll Sci Technol & Med, Dept Infect Dis, London SW7 2AZ, England
关键词
chitin; chitin synthesis; Aspergillus fumigatus; cell wall analysis;
D O I
10.1016/S1087-1845(02)00516-9
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Previous studies (Aufauvre-Brown et al., 1997; Mellado et al., 1996a,b) have shown that only two genes of the Aspergillus fumigatus chitin synthase family, chsG and chsE, play a role in the morphogenesis of this fungal species. An A. fumigatus strain lacking both chsG (class III CHS) and chsE (class V CHS) genes was constructed by gene replacement of the chsE gene with a copy that has its conserved coding region interrupted by the hph resistance cassette in an A. fumigatus ChsG(-) genetic background. Unexpectedly the double disruption was not lethal. The double mutant AfchsG(-)IchsE(-) strain (i) has reduced chitin synthase activity with or without trypsin stimulation, (ii) has a reduced colony radial growth rate, (iii) produces highly branched hyphae, (iv) exhibits aberrant features, such as periodic swellings along the length of the hyphae and a block in conidiation that can be partially restored by an osmotic stabilizer (v) shows alterations in the shape and germination capacity of the conidia, and (vi) has a cell wall that contains half the chitin of the parental strain and is, unexpectedly, highly enriched in alpha-(1-3) glucan. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
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页码:98 / 109
页数:12
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