Mapping protein abundance patterns in the brain using voxelation combined with liquid chromatography and mass spectrometry

被引：29

作者：

Petyuk, Vladislav A. ^{[2
,3
]}

Qian, Wei-Jun ^{[2
,3
]}

Smith, Richard D. ^{[2
,3
]}

Smith, Desmond J. ^{[1
]}

机构：

[1] Univ Calif Los Angeles, David Geffen Sch Med, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA

[2] Pacific NW Natl Lab, Div Biol Sci, Richland, WA 99352 USA

[3] Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA

来源：

METHODS | 2010年 / 50卷 / 02期

关键词：

Brain atlas; Brain mapping; Mass spectrometry; Proteomics; Transcriptomics; Voxelation; TIME TAG APPROACH; GENE-EXPRESSION; ACCURATE MASS; STATISTICAL-MODEL; NERVOUS-SYSTEM; IDENTIFICATIONS; PROTEOMICS; PEPTIDE; DATABASE; SEARCH;

D O I：

10.1016/j.ymeth.2009.07.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Voxelation creates expression atlases by high-throughput analysis of spatially registered cubes or voxels harvested from the brain. The modality independence of voxelation allows a variety of bioanalytical techniques to be used to map abundance. Protein expression patterns in the brain can be obtained using liquid chromatography (LC) combined with mass spectrometry (MS). Here we describe the methodology of voxelation as it pertains particularly to LC-MS proteomic analysis: sample preparation instrumental. set up and analysis, peptide identification and protein relative abundance quantitation. We also briefly describe some of the advantages, limitations and insights into the brain that can be obtained using combined proteomic and transcriptomic maps. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：77 / 84

页数：8

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