Morphology, Transcriptomics and In Vitro Model of Skin from Polar Cod (Boreogadus Saida) and Atlantic Cod (Gadus Morhua)

被引:13
作者
Ytteborg, Elisabeth [1 ]
Hansen, Oyvind Johannes [1 ]
Host, Vibeke [1 ]
Afanasyev, Sergey [2 ]
Vieweg, Ireen [3 ]
Nahrgang, Jasmine [3 ]
Krasnov, Aleksei [1 ]
机构
[1] Nofima, Muninbakken 9-13, N-9019 Tromso, Norway
[2] Russian Acad Sci, Sechenov Inst Evolutionary Physiol & Biochem, Torez 44, St Petersburg 194223, Russia
[3] UiT Arctic Univ Norway, Dept Arctic & Marine Biol, POB 6050, N-9037 Tromso, Langnes, Norway
关键词
atlantic cod; hydrogen peroxide; in vitro; keratocytes; polar cod; skin; SALMO-SALAR; HYDROGEN-PEROXIDE; MUCOUS CELLS; SHARK SKIN; TASTE-BUDS; FISH; RESPONSES; TEMPERATURE; ZEBRAFISH; STRESS;
D O I
10.3390/fishes5040034
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Fish skin is a multifunctional barrier tissue with high regeneration capacity that interacts with the surrounding environment and provides protection. Functional importance, high complexity and activity make skin an attractive tissue for studying the effects of environmental challenges and chemical stressors in fish. The aim of this work was to characterize skin from polar cod (Boreogadus saida) and Atlantic cod (Gadus morhua), and to test cod skin as an in vitro model in exposure studies. Both species have similar skin structures including epidermis, mucous cells, club cells and scales. However, microarchitectural differences were detected; Atlantic cod has a smooth epidermal surface and overlapping scales, whereas polar cod has a folded outer surface and discontinuous scales. Genome-wide microarray found 6.5k genes with expression differences, which suggested more active turnover of proteins, proliferation and motility of cells in skin of polar cod. Hydrogen peroxide (H2O2) was used to examine skin responses. Transcriptome response was stronger in the skin of polar cod, with 155 differentially expressed genes. The skin from Atlantic cod was further used to develop a cell culture. H2O2 decreased the cell migration rate in a dose-dependent manner, which could indicate reduced skin healing capacity. The results revealed novel skin structures and confirmed that the skin from cod is a promising tissue for evaluation of stressors.
引用
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页码:1 / 15
页数:15
相关论文
共 70 条
[41]   Body site matters: an evaluation and application of a novel histological methodology on the quantification of mucous cells in the skin of Atlantic salmon, Salmo salar L. [J].
Pittman, K. ;
Pittman, A. ;
Karlson, S. ;
Cieplinska, T. ;
Sourd, P. ;
Redmond, K. ;
Ravnoy, B. ;
Sweetman, E. .
JOURNAL OF FISH DISEASES, 2013, 36 (02) :115-127
[42]  
Quilhac A, 1999, ANAT REC, V254, P435
[43]   Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules [J].
Rajan, Binoy ;
Fernandes, Jorge M. O. ;
Caipang, Christopher M. A. ;
Kiron, Viswanath ;
Rombout, Jan H. W. M. ;
Brinchmann, Monica E. .
FISH & SHELLFISH IMMUNOLOGY, 2011, 31 (02) :224-231
[44]   Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing [J].
Rapanan, Jose L. ;
Pascual, Agnes S. ;
Uppalapati, Chandana K. ;
Cooper, Kimbal E. ;
Leyva, Kathryn J. ;
Hull, Elizabeth E. .
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2015, (96)
[45]   Collective cell migration of primary zebrafish keratocytes [J].
Rapanan, Jose L. ;
Cooper, Kimbal E. ;
Leyva, Kathryn J. ;
Hull, Elizabeth E. .
EXPERIMENTAL CELL RESEARCH, 2014, 326 (01) :155-165
[46]   BRUTONS TYROSINE KINASE IS A KEY REGULATOR IN B-CELL DEVELOPMENT [J].
RAWLINGS, DJ ;
WITTE, ON .
IMMUNOLOGICAL REVIEWS, 1994, 138 :105-119
[47]   Influences of thermal acclimation and acute temperature change on the motility of epithelial wound-healing cells (keratocytes) of tropical, temperate and Antarctic fish [J].
Ream, RA ;
Theriot, JA ;
Somero, GN .
JOURNAL OF EXPERIMENTAL BIOLOGY, 2003, 206 (24) :4539-4551
[48]   Adult Zebrafish as a Model System for Cutaneous Wound-Healing Research [J].
Richardson, Rebecca ;
Slanchev, Krasimir ;
Kraus, Christopher ;
Knyphausen, Philipp ;
Eming, Sabine ;
Hammerschmidt, Matthias .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2013, 133 (06) :1655-1665
[49]   Calcium mobilization from fish scales is mediated by parathyroid hormone related protein via the parathyroid hormone type 1 receptor [J].
Rotllant, J ;
Redruello, B ;
Guerreiro, PM ;
Fernandes, H ;
Canario, AVM ;
Power, DM .
REGULATORY PEPTIDES, 2005, 132 (1-3) :33-40
[50]   Thermal reception in fishes [J].
Rubin, MA .
JOURNAL OF GENERAL PHYSIOLOGY, 1935, 18 (05) :643-647