Quantitative analysis of flavonoids by reversed-phase high-performance liquid chromatography

被引：142
作者：
Crozier, A
Jensen, E
Lean, MEJ
McDonald, MS
机构：
[1] UNIV TROMSO,DEPT PHARMACOL,N-9037 TROMSO,NORWAY
[2] UNIV GLASGOW,ROYAL INFIRM,DEPT HUMAN NUTR,GLASGOW G31 2ER,LANARK,SCOTLAND
来源：
JOURNAL OF CHROMATOGRAPHY A | 1997年 / 761卷 / 1-2期
关键词：
plant extracts; flavonoids;
D O I：
10.1016/S0021-9673(96)00826-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Large variations were found in the effectiveness of five reversed-phase high-performance liquid chromatography columns used for the analysis of free and conjugated flavonoids. The best results were obtained with C-18 Nova-Pak, C-18 Symmetry and C-18 Genesis columns but substantial band broadening and peak asymmetry were observed when free flavonoids were chromatographed on ODS-Hypersil and LiChrospher RP-18 supports. The Symmetry and Genesis columns provided gradient elution separations of rutin, quercetin-3-glucoside, quercitrin, myricetin, luteolin, quercetin, apigenin, kaempferol and isorhamnetin. This procedure was used for the quantitative analysis of endogenous flavones and flavonols in acid hydrolysed extracts from onions and celery.
引用
收藏
页码：315 / 321
页数：7
相关论文
共 19 条
[1]   FLAVONOID ACTIVATION OF NODULATION GENES IN RHIZOBIUM REVERSED BY OTHER COMPOUNDS PRESENT IN PLANTS [J].
FIRMIN, JL ;
WILSON, KE ;
ROSSEN, L ;
JOHNSTON, AWB .
NATURE, 1986, 324 (6092) :90-92
[2]  
Gregory J, 1990, DIETARY NUTR SURVEY
[3]  
Harborne JB, 1994, ADV RES 1986
[4]   ON THE OCCURRENCE OF FLAVONOL AND FLAVONE GLYCOSIDES IN VEGETABLES [J].
HERRMANN, K .
ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG, 1988, 186 (01) :1-5
[5]  
Herrmann K., 1976, Journal of Food Technology, V11, P433, DOI 10.1108/09566160010378107
[6]  
Hertog M. G. L., 1994, THESIS AGR U WAGENIN
[7]   DIETARY ANTIOXIDANT FLAVONOIDS AND RISK OF CORONARY HEART-DISEASE - THE ZUTPHEN ELDERLY STUDY [J].
HERTOG, MGL ;
FESKENS, EJM ;
HOLLMAN, PCH ;
KATAN, MB ;
KROMHOUT, D .
LANCET, 1993, 342 (8878) :1007-1011
[8]   CONTENT OF POTENTIALLY ANTICARCINOGENIC FLAVONOIDS OF 28 VEGETABLES AND 9 FRUITS COMMONLY CONSUMED IN THE NETHERLANDS [J].
HERTOG, MGL ;
HOLLMAN, PCH ;
KATAN, MB .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1992, 40 (12) :2379-2383
[9]   CONTENT OF POTENTIALLY ANTICARCINOGENIC FLAVONOIDS OF TEA INFUSIONS, WINES, AND FRUIT JUICES [J].
HERTOG, MGL ;
HOLLMAN, PCH ;
VANDEPUTTE, B .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1993, 41 (08) :1242-1246
[10]   THE FLAVONOID BIOSYNTHETIC-PATHWAY IN PLANTS - FUNCTION AND EVOLUTION [J].
KOES, RE ;
QUATTROCCHIO, F ;
MOL, JNM .
BIOESSAYS, 1994, 16 (02) :123-132
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