Defective interaction between Pol2p and Dpb2p, subunits of DNA polymerase epsilon, contributes to a mutator phenotype in Saccharomyces cerevisiae

被引：13

作者：

Jaszczur, Malgorzata ^{[1
]}

Rudzka, Justyna ^{[1
]}

Kraszewska, Joanna ^{[1
]}

Flis, Krzysztof ^{[1
]}

Polaczek, Piotr ^{[2
]}

Campbell, Judith L. ^{[2
]}

Fijalkowska, Iwona J. ^{[1
]}

Jonczyk, Piotr ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Biochem & Biophys, PL-02106 Warsaw, Poland

[2] CALTECH, Braun Lab, Pasadena, CA 91125 USA

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2009年 / 669卷 / 1-2期

关键词：

DNA polymerase; Polymerase epsilon holoenzyme (Pol epsilon HE); Spontaneous mutagenesis; Fidelity of DNA replication; Protein-protein interaction; DPB2 (YPR175W) and POL2 (YNL262W) genes of the yeast Saccharomyces cerevisiae; PROTEIN-PROTEIN INTERACTIONS; ESCHERICHIA-COLI; I GENE; YEAST; REPLICATION; MUTANTS; MUTATIONS; COMPLEX; CLONING; DELTA;

D O I：

10.1016/j.mrfmmm.2009.04.012

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Most of the prokaryotic and eukaryotic replicative polymerases are multi-subunit complexes. There are several examples indicating that noncatalytic subunits of DNA polymerases may function as fidelity factors; during replication process In this work, we have further investigated the role of Dpb2p, a noncatalytic subunit of DNA polymerase epsilon holoenzyme from Saccharomyces cerevisiae in controlling the level of spontaneous mutagenesis The data presented indicate that impaired interaction between catalytic Pol2p subunit and Dpb2p is responsible for the observed mutator phenotype in S. cerevisiae strains carrying different mutated alleles of the DPB2 gene. We observed a significant correlation between the decreased level of interaction between different mutated forms of Dpb2p towards a wild-type form of Pol2p and the strength Of mutator phenotype that they confer We propose that structural integrity of the Pol epsilon holoenzyme is essential for genetic stability in S cerevisiae cells. (C) 2009 Elsevier B.V. All rights reserved

引用

页码：27 / 35

页数：9

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