Maximizing DNA loading on a range of gold nanoparticle sizes

被引：997
作者：
Hurst, Sarah J.
Lytton-Jean, Abigail K. R.
Mirkin, Chad A.
机构：
[1] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA
[2] Northwestern Univ, Int Inst Nanotechnol, Evanston, IL 60208 USA
来源：
ANALYTICAL CHEMISTRY | 2006年 / 78卷 / 24期
关键词：
D O I：
10.1021/ac0613582
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We have investigated the variables that influence DNA coverage on gold nanoparticles. The effects of salt concentration, spacer composition, nanoparticle size, and degree of sonication have been evaluated. Maximum loading was obtained by salt aging the nanoparticles to similar to 0.7 M NaCl in the presence of DNA containing a poly( ethylene glycol) spacer. In addition, DNA loading was substantially increased by sonicating the nanoparticles during the surface loading process. Last, nanoparticles up to 250 nm in diameter were found have similar to 2 orders of magnitude higher DNA loading than smaller (13-30 nm) nanoparticles, a consequence of their larger surface area. Stable large particles are attractive for a variety of biodiagnostic assays.
引用
收藏
页码：8313 / 8318
页数：6
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