Midpregnancy testing for soluble fms-like tyrosine kinase 1 (sFlt-1) and placental growth factor (PlGF): An inter-assay comparison of three automated immunoassay platforms

被引：3

作者：

Black, Carin ^{[1
,2
]}

Al-Amin, Ahmed ^{[5
,6
]}

Rolnik, Daniel Lorber ^{[4
,5
]}

Kane, Stefan C. ^{[1
,2
,6
]}

Stolarek, Caroline ^{[1
]}

White, Adrienne ^{[1
]}

Costa, Fabricio da Silva ^{[3
,4
]}

Brennecke, Shaun ^{[1
,2
]}

机构：

[1] Royal Womens Hosp, Dept Maternal Fetal Med, Pregnancy Res Ctr, 20 Flemington Rd, Parkville, Vic 3052, Australia

[2] Univ Melbourne, Dept Obstet & Gynaecol, Royal Womens Hosp, 20 Flemington Rd, Parkville, Vic 3052, Australia

[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Gynecol & Obstet, Ribeirao Preto, SP, Brazil

[4] Monash Med Ctr, Perinatal Serv, 246 Clayton Rd, Clayton, Vic 3168, Australia

[5] Monash Univ, Dept Obstet & Gynaecol, Wellington Rd, Clayton, Vic 3800, Australia

[6] Royal Womens Hosp, Pauline Gandel Imaging Ctr, 20 Flemington Rd, Parkville, Vic 3052, Australia

来源：

PLACENTA | 2019年 / 86卷

关键词：

ANGIOGENIC FACTORS; PREECLAMPSIA; ISOFORM; RISK;

D O I：

10.1016/j.placenta.2019.08.083

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We performed an inter-assay comparison among three immunoassay platforms for midpregnancy testing of sFlt-1, PlGF and the sFlt-1/PlGF ratio, which are established markers for pre-eclampsia. Maternal blood was collected 19-22 weeks' gestation. Raw data values were converted to multiples of the median (MoM). PlGF and sFlt-1 values among platforms were highly correlated (p < 0.001). There was significant variation in raw data values for PlGF and sFlt-1 among platforms, eliminated following conversion to MoM. When directly comparing raw data values among platforms, platform-specific reference ranges values should be used. MoM values were equivalent among platforms, allowing direct inter-assay result comparison.

引用

页码：11 / 14

页数：4

共 18 条

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