Innovative approach for urease inhibition by Ficus carica extract-fabricated silver nanoparticles: An in vitro study

被引：26

作者：

Borase, Hemant P. ^{[1
]}

Salunkhe, Rahul B. ^{[1
]}

Patil, Chandrashekhar D. ^{[1
]}

Suryawanshi, Rahul K. ^{[1
]}

Salunke, Bipinchandra K. ^{[1
]}

Wagh, Nilesh D. ^{[2
]}

Patil, Satish V. ^{[1
,3
]}

机构：

[1] North Maharashtra Univ, Sch Life Sci, Jalgaon 425001, Maharashtra, India

[2] North Maharashtra Univ, Sch Environm Sci, Jalgaon, Maharashtra, India

[3] North Maharashtra Univ, North Maharashtra Microbial Culture Collect Ctr N, Jalgaon, Maharashtra, India

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2015年 / 62卷 / 06期

关键词：

biomaterials; FT-IR; Helicobacter pylori; silver nanoparticles; urease inhibitors; GRAM-NEGATIVE BACTERIA; RESISTANCE; BIOSYNTHESIS; NITRATE; LATEX;

D O I：

10.1002/bab.1341

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, a rapid, low-cost, and ecofriendly method of stable silver nanoparticles (AgNPs) synthesis using leaves extract of Ficus carica (F.carica), a plant with diverse metabolic consortium, is reported for the first time. An absorption peakat 422nm in UV-Vis spectroscopy, a spherical shape with an average size of 21nm in transmission electron microscopy, and crystalline nature in X-ray powder diffraction studies were observed for the synthesized AgNPs. Fourier transform infrared analysis indicated that proteins of F.carica might have a vital role in AgNP synthesis and stabilization. AgNPs were found to inhibit urease, a key enzyme responsible for the survival and pathogenesis of the bacterium, Helicobacter pylori. Inhibition of urease by AgNPs was monitored spectrophotometrically by the evaluation of ammonia release. The urease inhibition potential of AgNPs can be explored in the treatment of H. pylori by preparing novel combinations of standard drugs with AgNPs- or AgNPs-encapsulated drug molecules. (C) 2015 International Union of Biochemistry and Molecular Biology, Inc.

引用

页码：780 / 784

页数：5

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