Bioluminescence Imaging in Mouse Models Quantifies β Cell Mass in the Pancreas and After Islet Transplantation

被引：63

作者：

Virostko, John ^{[2
,3
]}

Radhika, Aramandla ^{[1
]}

Poffenberger, Greg ^{[1
]}

Chen, Zhongyi ^{[1
]}

Brissova, Marcela ^{[1
]}

Gilchrist, Joshua ^{[1
]}

Coleman, Brian ^{[1
]}

Gannon, Maureen ^{[1
,4
,5
]}

Jansen, E. Duco ^{[3
]}

Powers, Alvin C. ^{[1
,4
,6
]}

机构：

[1] Vanderbilt Univ, Dept Med, Div Endocrinol Diabet & Metab, Nashville, TN 37203 USA

[2] Vanderbilt Univ, Inst Imaging Sci, Nashville, TN USA

[3] Vanderbilt Univ, Dept Biomed Engn, Nashville, TN 37235 USA

[4] Vanderbilt Univ, Dept Mol Physiol & Biophys, Nashville, TN 37232 USA

[5] Vanderbilt Univ, Dept Cell & Dev Biol, Nashville, TN USA

[6] VA Tennessee Valley Healthcare Syst, Nashville, TN USA

来源：

MOLECULAR IMAGING AND BIOLOGY | 2010年 / 12卷 / 01期

基金：

美国国家卫生研究院;

关键词：

Bioluminescence imaging; Luciferase; Beta cell; Pancreatic islet; Transplantation; Optical imaging; Islet mass; Beta cell mass; Imaging; POSITRON-EMISSION-TOMOGRAPHY; DIABETIC NUDE-MICE; IN-VIVO; INSULIN-SECRETION; GENE-EXPRESSION; GRAFT-SURVIVAL; GLUCOSE; RAT; TRANSCRIPTION; TOLERANCE;

D O I：

10.1007/s11307-009-0240-1

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

We developed a mouse model that enables non-invasive assessment of changes in beta cell mass. We generated a transgenic mouse expressing luciferase under control of the mouse insulin I promoter [mouse insulin promoter-luciferase-Vanderbilt University (MIP-Luc-VU)] and characterized this model in mice with increased or decreased beta cell mass and after islet transplantation. Streptozotocin-induced, diabetic MIP-Luc-VU mice had a progressive decline in bioluminescence that correlated with a decrease in beta cell mass. MIP-Luc-VU animals fed a high-fat diet displayed a progressive increase in bioluminescence that reflected an increase in beta cell mass. MIP-Luc-VU islets transplanted beneath the renal capsule or into the liver emitted bioluminescence proportional to the number of islets transplanted and could be imaged for more than a year. Bioluminescence in the MIP-Luc-VU mouse model is proportional to beta cell mass in the setting of increased and decreased beta cell mass and after transplantation.

引用

页码：42 / 53

页数：12

共 49 条

[1] Magnetic resonance-guided, real-time targeted delivery and imaging of magnetocapsules immunoprotecting pancreatic islet cells [J].

Barnett, Brad P. ;

Arepally, Aravind ;

Karmarkar, Parag V. ;

Qian, Di ;

Gilson, Wesley D. ;

Walczak, Piotr ;

Howland, Valerie ;

Lawler, Leo ;

Lauzon, Cal ;

Stuber, Matthias ;

Kraitchman, Dara L. ;

Bulte, Jeff W. M. .

NATURE MEDICINE, 2007, 13 (08) :986-991

[2] Magnetic resonance imaging of gadolinium-labeled pancreatic islets for experimental transplantation [J].

Biancone, Luigi ;

Crich, Simonetta Geninatti ;

Cantaluppi, Vincenzo ;

Romanazzi, Giuseppe Mauriello ;

Russo, Simona ;

Scalabrino, Elisa ;

Esposito, Giovanna ;

Figliolini, Federico ;

Beltramo, Silvia ;

Perin, Paolo Cavallo ;

Segoloni, Giuseppe Paolo ;

Aime, Silvio ;

Camussi, Giovanni .

NMR IN BIOMEDICINE, 2007, 20 (01) :40-48

[3] PARTIAL PANCREATECTOMY IN THE RAT AND SUBSEQUENT DEFECT IN GLUCOSE-INDUCED INSULIN RELEASE [J].

BONNERWEIR, S ;

TRENT, DF ;

WEIR, GC .

JOURNAL OF CLINICAL INVESTIGATION, 1983, 71 (06) :1544-1553

[4] Assessment of human pancreatic islet architecture and composition by laser scanning confocal microscopy [J].

Brissova, M ;

Fowler, MJ ;

Nicholson, WE ;

Chu, A ;

Hirshberg, B ;

Harlan, DM ;

Powers, AC .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2005, 53 (09) :1087-1097

[5] Reduced PDX-1 expression impairs islet response to insulin resistance and worsens glucose homeostasis [J].

Brissova, M ;

Blaha, M ;

Spear, C ;

Nicholson, W ;

Radhika, A ;

Shiota, M ;

Charron, MJ ;

Wright, CVE ;

Powers, AC .

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2005, 288 (04) :E707-E714

[6] Intraislet endothelial cells contribute to revascularization of transplanted pancreatic islets [J].

Brissova, M ;

Fowler, M ;

Wiebe, P ;

Shostak, A ;

Shiota, M ;

Radhika, A ;

Lin, PC ;

Gannon, M ;

Powers, AC .

DIABETES, 2004, 53 (05) :1318-1325

[7] Reduction in pancreatic transcription factor PDX-1 impairs glucose-stimulated insulin secretion [J].

Brissova, M ;

Shiota, M ;

Nicholson, WE ;

Gannon, M ;

Knobel, SM ;

Piston, DW ;

Wright, CVE ;

Powers, AC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (13) :11225-11232

[8] In vivo bioluminescence imaging of transplanted islets and early detection of graft rejection [J].

Chen, Xiaojuan ;

Zhang, Xiaomin ;

Larson, Courtney S. ;

Baker, Marshall S. ;

Kaufman, Dixon B. .

TRANSPLANTATION, 2006, 81 (10) :1421-1427

[9] Neurofunctional imaging of the pancreas utilizing the cholinergic PET radioligand [18F]4-fluorobenzyltrozamicol [J].

Clark, PB ;

Gage, HD ;

Brown-Proctor, C ;

Buchheimer, N ;

Calles-Escandon, J ;

Mach, RH ;

Morton, KA .

EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 2004, 31 (02) :258-260

[10] Visualizing gene expression in living mammals using a bioluminescent reporter [J].

Contag, CH ;

Spilman, SD ;

Contag, PR ;

Oshiro, M ;

Eames, B ;

Dennery, P ;

Stevenson, DK ;

Benaron, DA .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1997, 66 (04) :523-531

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