MAPK upstream kinase (MUK)-binding inhibitory protein, a negative regulator of MUK/dual leucine zipper-bearing kinase/leucine zipper protein kinase

被引:37
作者
Fukuyama, K
Yoshida, M
Yamashita, A
Deyama, T
Baba, M
Suzuki, A
Mohri, H
Ikezawa, Z
Nakajima, H
Hirai, S
Ohno, S
机构
[1] Yokohama City Univ, Sch Med, Dept Mol Biol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
[2] Yokohama City Univ, Sch Med, Dept Dermatol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
[3] Yokohama City Univ, Sch Med, Dept Internal Med 1, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
[4] Yokohama City Univ, Sch Med, Dept Urol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
关键词
D O I
10.1074/jbc.M001488200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mitogen-activated protein kinase upstream kinase/dual leucine zipper-bearing kinase/leucine-zipper protein kinase (MUK/DLK/ZPK) is a MAPKKK class protein kinase that induces JNK/SAPK activation. We report here a protein named MBIP that binds to MUK/DLK/ZPK. MUK-binding inhibitory protein (MBIP) contains two tandemly orientated leucine-zipper-like motifs with a cluster of basic amino acids located between the two motifs. MBIP interacts with one of the two leucine-zipper-like motifs of MUK/DLK/ZPK and inhibits the activity of MUK/DLK/ZPK to induce JNK/SAPK activation. Notably, no similar effect was observed with another JNK/SAPK-inducing MAPKKK, COT/Tp1-2, showing the specificity of MBIP action. Furthermore, the overexpression of MBIP partially inhibits the activation of JNK by 0.3 M sorbitol in 293T cells. Taken together, these observations indicate that MBIP can function as a regulator of MUK/DLK/ZPK, a finding that may provide a clue to understanding the molecular mechanism of JNK/SAPK activation by hyperosmotic stress.
引用
收藏
页码:21247 / 21254
页数:8
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共 45 条
  • [1] Inhibition of cell growth by overexpression of the ZPK gene
    Bergeron, P
    Douziech, M
    Daigle, R
    Blouin, R
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 231 (01) : 153 - 155
  • [2] Cell-specific expression of the ZPK gene in adult mouse tissues
    Blouin, R
    Beaudoin, J
    Bergeron, P
    Nadeau, A
    Grondin, G
    [J]. DNA AND CELL BIOLOGY, 1996, 15 (08) : 631 - 642
  • [3] A CONSERVED BINDING MOTIF DEFINES NUMEROUS CANDIDATE TARGET PROTEINS FOR BOTH CDC42 AND RAC GTPASES
    BURBELO, PD
    DRECHSEL, D
    HALL, A
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (49) : 29071 - 29074
  • [4] The regulation of anoikis: MEKK-1 activation requires cleavage by caspases
    Cardone, MH
    Salvesen, GS
    Widmann, C
    Johnson, G
    Frisch, SM
    [J]. CELL, 1997, 90 (02) : 315 - 323
  • [5] Persistent activation of c-Jun N-terminal kinase 1 (JNK1) in gamma radiation-induced apoptosis
    Chen, YR
    Meyer, CF
    Tan, TH
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (02) : 631 - 634
  • [6] IDENTIFICATION OF A NEW FAMILY OF HUMAN EPITHELIAL PROTEIN-KINASES CONTAINING 2 LEUCINE ISOLEUCINE-ZIPPER DOMAINS
    DOROW, DS
    DEVEREUX, L
    DIETZSCH, E
    DEKRETSER, T
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 213 (02): : 701 - 710
  • [7] Localization of the mixed-lineage kinase DLK/MUK/ZPK to the Golgi apparatus in NIH 3T3 cells
    Douziech, M
    Laberge, G
    Grondin, G
    Daigle, N
    Blouin, R
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1999, 47 (10) : 1287 - 1296
  • [8] Zonal induction of mixed lineage kinase ZPK/DLK/MUK gene expression in regenerating mouse liver
    Douziech, M
    Grondin, G
    Loranger, A
    Marceau, N
    Blouin, R
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 249 (03) : 927 - 932
  • [9] Dual leucine zipper-bearing kinase (DLK) activates p46(SAPK) and p38(mapk) but not ERK2
    Fan, G
    Merritt, SE
    Kortenjann, M
    Shaw, PE
    Holzman, LB
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (40) : 24788 - 24793
  • [10] AN OSMOSENSING SIGNAL-TRANSDUCTION PATHWAY IN MAMMALIAN-CELLS
    GALCHEVAGARGOVA, Z
    DERIJARD, B
    WU, IH
    DAVIS, RJ
    [J]. SCIENCE, 1994, 265 (5173) : 806 - 808