Flow cytometric analysis for adhesion of Vibrio cholerae to human intestinal epithelial cell

被引：3
作者：
Taguchi, H
Yamaguchi, H
Osaki, TY
Yamamoto, T
Ogata, S
Kamiya, S
机构：
[1] Department of Microbiology, Kyorin University, School of Medicine, Mitaka, Tokyo, Shinkawa
[2] Divison of Flow Cytometry, Kyorin University, School of Medicine, Mitaka, Tokyo, Shinkawa
[3] Department of Microbiology, Kyorin University, School of Medicine, Mitaka, Tokyo 181
来源：
EUROPEAN JOURNAL OF EPIDEMIOLOGY | 1997年 / 13卷 / 06期
关键词：
adhesion; flow cytometry; intestinal epithelial cell; Vibrio cholerae;
D O I：
10.1023/A:1007352713686
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
The adhesion of Vibrio cholerae O1 strains to human intestinal epithelial cell, Intestine 407, was analyzed by flow cytometer. According to positive percentages of Intestine 407 cells adhered by V. cholerae, two groups of V. cholerae strains were classified as follows: more adhesive (more than 50%), less adhesive (less than 50%) strains. In addition, the fluorescence intensity after attachment of V. cholerae was directly correlated to the number of the microorganisms. It was concluded that flow cytometry is a useful and objective method for analyzing adhesion of V. cholerae to cultured cells.
引用
收藏
页码：719 / 724
页数：6
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