Microwave-Assisted Lipase Covalent Immobilization and Improved Its Thermal Stability Using Alcohols to Quench Excessive Activated Groups on Support Surface

被引：4

作者：

Du, Zhiqiang ^{[1
]}

Wang, Anming ^{[1
]}

Zhou, Cheng ^{[1
]}

Zhu, Shemin ^{[2
]}

Shen, Shubao ^{[1
]}

机构：

[1] Nanjing Univ Technol, Coll Biotechnol & Pharmaceut Engn, Nanjing 210009, Peoples R China

[2] Nanjing Univ Technol, Coll Mat Sci & Engn, Nanjing 210009, Peoples R China

来源：

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN | 2009年 / 42卷 / 06期

关键词：

Immobilization; Microwave Irradiation; Quench; Blockage; Mesocellular Siliceous Foams; MESOPOROUS SILICA; PENICILLIN ACYLASE; ENZYMES; EFFICIENT; RESOLUTION; ATTACHMENT; INCREASE;

D O I：

10.1252/jcej.08we304

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Microwave irradiation technology is used to covalently immobilize lipase at low temperature to improve enzyme properties. The specific activity of lipase immobilized under microwave irradiation was 114.4 U.mg(-1), 1.23 times of that for a traditional method without microwave irradiation. The K-m and K-cat/K-m of microwave immobilized lipase was 0.12 and 1.26 folds that of free lipase. Moreover, alcohols were used to quench the excessive activated groups on MCFs surface to improve thermal stability of immobilized lipase. Residual activity of immobilized lipase quenched by ethanol, retained 60.64% after incubation at 50 degrees C for 5 h, 2.36 and 1.99 times higher than those of free lipase and immobilized lipase which was not quenched by alcohols, respectively. The results showed that microwave irradiation could improve lipase covalent immobilization and quenching remaining groups could enhance thermal stability of immobilized lipase.

引用

页码：441 / 446

页数：6