Ferulic Acid Regulates the Nrf2/Heme Oxygenase-1 System and Counteracts Trimethyltin-Induced Neuronal Damage in the Human Neuroblastoma Cell Line SH-SY5Y

被引:90
作者
Catino, Stefania [1 ]
Paciello, Fabiola [2 ]
Miceli, Fiorella [1 ]
Rolesi, Rolando [2 ]
Troiani, Diana [3 ]
Calabrese, Vittorio [4 ]
Santangelo, Rosaria [5 ]
Mancuso, Cesare [1 ]
机构
[1] Catholic Univ, Sch Med, Inst Pharmacol, Rome, Italy
[2] Catholic Univ, Sch Med, Dept Head & Neck Surg, Rome, Italy
[3] Catholic Univ, Sch Med, Inst Human Physiol, Rome, Italy
[4] Univ Catania, Sch Med, Dept Biomed & Biotechnol Sci, Catania, Italy
[5] Catholic Univ, Sch Med, Inst Microbiol, Rome, Italy
关键词
biliverdin reductase; cell stress response; ferulic acid; heme oxygenase; Nrf2; SH-SY5Y; trimethyltin; HUMAN BILIVERDIN REDUCTASE; SOLID LIPID NANOPARTICLES; RAT-BRAIN MICROSOMES; OXIDATIVE STRESS; HEME OXYGENASE; CARBON-MONOXIDE; ETHYL-ESTER; NEURODEGENERATIVE DISORDERS; NUCLEAR TRANSLOCATION; PROSTATE-CANCER;
D O I
10.3389/fphar.2015.00305
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Over the past years, several lines of evidence have pointed out the efficacy of ferulic acid (FA) in counteracting oxidative stress elicited by beta-amyloid or free radical initiators, based on the ability of this natural antioxidant to up regulate the heme oxygenase-1 (HO-1) and biliverdin reductase (BVR) system. However, scarce results can be found in literature regarding the cytoprotective effects of FA in case of damage caused by neurotoxicants. The aim of this work is to investigate the mechanisms through which FA exerts neuroprotection in SH-SY5Y neuroblastoma cells exposed to the neurotoxin trimethyltin (TMT). FA (1-10 mu M for 6 h) dose-dependently increased both basal and TMT (10 mu M for 24 h)-induced HO-1 expression in SH-SY5Y cells by fostering the nuclear translocation of the transcriptional activator Nrf2. In particular, the co-treatment of FA (10 mu M) with TMT was also responsible for the nuclear translocation of HO-1 in an attempt to further increase cell stress response in SH-SY5Y cells. In addition to HO-1, FA (1-10 mu M for 6 h) dose-dependently increased the basal expression of BVR. The antioxidant and neuroprotective features of FA, through the increase of HO activity, were supported by the evidence that FA inhibited TMT (10 mu M)-induced lipid peroxidation (evaluated by detecting 4-hydroxy-nonenal) and DNA fragmentation in SH-SY5Y cells and that this antioxidant effect was reversed by the HO inhibitor Zinc-protoporphyrin-IX (5 mu M). Among the by-products of the HO/BVR system, carbon monoxide (CORM-2, 50 nM) and bilirubin (BR, 50 nM) significantly inhibited TMT-induced superoxide anion formation in SH-SY5Y cells. All together, these results corroborate the neuroprotective effect of FA through the up-regulation of the HO-1/BVR system, via carbon monoxide and BR formation, and provide the first evidence on the role of HO-1/Nrf2 axis in FA-related enhancement of cell stress response in human neurons.
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页数:12
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