Fate of liberated viral RNA in wastewater determined by PCR

被引:45
作者
Limsawat, S [1 ]
Ohgaki, S [1 ]
机构
[1] UNIV TOKYO,FAC ENGN,DEPT URBAN ENGN,BUNKYO KU,TOKYO 113,JAPAN
关键词
D O I
10.1128/AEM.63.7.2932-2933.1997
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The use of a PCR method to investigate the fate of liberated Q beta coliphage RNA in wastewater suggests that a positive PCR result indicates the recent presence of potentially viable virus in the sample and therefore increases the public health significance of the result.
引用
收藏
页码:2932 / 2933
页数:2
相关论文
共 50 条
[21]   Fate and mass balance of contaminants of emerging concern during wastewater treatment determined using the fractionated approach [J].
Baalbaki, Zeina ;
Sultana, Tamanna ;
Maere, Thomas ;
Vanrolleghem, Peter A. ;
Metcalfe, Chris D. ;
Yargeau, Viviane .
SCIENCE OF THE TOTAL ENVIRONMENT, 2016, 573 :1147-1158
[22]   Degradation of viral RNA in wastewater complex matrix models and other standards for wastewater-based epidemiology: A review [J].
Parra-Arroyo, Lizeth ;
Martinez-Ruiz, Manuel ;
Lucero, Sofia ;
Oyervides-Munoz, Mariel A. ;
Wilkinson, Mollie ;
Melchor-Martinez, Elda M. ;
Araujo, Rafael G. ;
Coronado-Apodaca, Karina G. ;
Velasco Bedran, Hugo ;
Buitron, German ;
Noyola, Adalberto ;
Barcelo, Damia ;
Iqbal, Hafiz M. N. ;
Eduardo Sosa-Hernandez, Juan ;
Parra-Saldivar, Roberto .
TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2023, 158
[23]   Efficient extraction of viral DNA and viral RNA by the chemagic viral DNA/RNA kit allows sensitive detection of cytomegalovirus, hepatitis B virus, and hepatitis G virus by PCR [J].
Kleines, M ;
Schellenberg, K ;
Ritter, K .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (11) :5273-5276
[24]   CROONIAN LECTURE 1964 - RELEASE AND FATE OF TRANSMITTER LIBERATED BY ADRENERGIC NERVES [J].
BROWN, L .
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1965, 162 (986) :1-+
[25]   Sensitivity and specificity of polyoma viral cytopathic effect in urine cytology samples as a function of viral load as determined by quantitative PCR for BK virus [J].
Steinman, S. L. ;
Stewart, J. .
MODERN PATHOLOGY, 2007, 20 :85A-85A
[26]   Sensitivity and specificity of polyoma viral cytopathic effect in urine cytology samples as a function of viral load as determined by quantitative PCR for BK virus [J].
Steinman, S. L. ;
Stewart, J. .
LABORATORY INVESTIGATION, 2007, 87 :85A-85A
[27]   Wastewater Surveillance of SARS-CoV-2 at a Canadian University Campus and the Impact of Wastewater Characteristics on Viral RNA Detection [J].
Bitter, Lena Carolin ;
Kibbee, Richard ;
Jimenez, Gabriela C. ;
Ormeci, Banu .
ACS ES&T WATER, 2022, 2 (11) :2034-2046
[28]   Storage of bovine viral diarrhoea virus samples on filter paper and detection of viral RNA by a RT-PCR method [J].
Vilcek, S ;
Strojny, L ;
Durkovic, B ;
Rossmanith, W ;
Paton, D .
JOURNAL OF VIROLOGICAL METHODS, 2001, 92 (01) :19-22
[29]   Absolute quantification of Bovine Viral Diarrhea Virus (BVDV) RNA by the digital PCR technique [J].
Flatschart, R. B. ;
Almeida, D. O. ;
Heinemann, M. B. ;
Medeiros, M. N. ;
Granjeiro, J. M. ;
Folgueras-Flatschart, A. V. .
VII BRAZILIAN CONGRESS ON METROLOGY (METROLOGIA 2013), 2015, 575
[30]   Quantification of HIV-1 viral RNA and proviral DNA by isotopic competitive PCR [J].
Gratzl, S ;
Moroni, C ;
Hirsch, HH .
JOURNAL OF VIROLOGICAL METHODS, 1997, 66 (02) :269-282